Fluorescence quenching of IAF-Na+/K(+)-ATPase via energy transfer to TNP-labeled nucleotide.
Fluorescence quenching of IAF-Na+/K(+)-ATPase via energy transfer to TNP-labeled nucleotide.
复制标题
通过能量转移至 TNP 标记的核苷酸来猝灭 IAF-Na /K( )-ATP 酶的荧光。
DOI：
10.1111/j.1749-6632.1997.tb52293.x
复制
发表时间：
1997
影响因子：
5.2
通讯作者：
Pratap,PR
中科院分区：
文献类型：
--
作者：
Hellen,EH;Pratap,PR
材料和方法-ADP和IAF来自分子探测器(俄勒冈州尤金)；其他化学品来自Sigma(圣路易斯，密苏里州)。冷冻的狗肾来自Pel-Freez(阿肯色州罗杰斯)。如前所述，获得了IAF标记的Na+/K+-ATPase。用于荧光测量的3-5种酶的浓度约为10 nm。缓冲液包括25 mM咪唑、pH 7.0、140 mM氯化胆碱、20 mM NaCI和1 mM EDTA。
MATERIALS AND METHODSTNP-ADP and IAF were from Molecular Probes (Eugene, Oregon); other chemicals were from Sigma (St. Louis, Missouri). Frozen dog kidneys were from Pel-Freez (Rogers, Arkansas). IAF-labeled Na+/K+-ATPase was obtained as described earlIer. 3-5 Enzyme concentrations used for fluorescence measurements were about 10 nM. The buffer contained 25 mM imidizole, pH 7.0, 140 mM choline chloride, 20 mM NaCI, and 1 mM EDTA.