TEMPORAL DIFFERENTIATION AND MIGRATION OF SUBSTANCE-P, SEROTONIN, AND SECRETIN IMMUNOREACTIVE ENTEROENDOCRINE CELLS IN THE MOUSE PROXIMAL SMALL-INTESTINE
TEMPORAL DIFFERENTIATION AND MIGRATION OF SUBSTANCE-P, SEROTONIN, AND SECRETIN IMMUNOREACTIVE ENTEROENDOCRINE CELLS IN THE MOUSE PROXIMAL SMALL-INTESTINE
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DOI：
10.1002/aja.1001940406
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发表时间：
1992-08-01
影响因子：
2.5
通讯作者：
ROTH, KA
中科院分区：
文献类型：
--
作者：
AIKEN, KD;ROTH, KA
小鼠胃肠道中的 P 物质、血清素和含促胰液素的肠内分泌细胞之间存在精确的空间相互关系。在近端小肠中，这些产物在沿着隐窝到绒毛轴的单个肠内分泌细胞中以各种组合共表达，其模式表明 P 物质、血清素和促胰液素的顺序表达。在本报告中，我们使用溴脱氧尿苷 (BrdU) 和多标记免疫组织化学来定义小鼠近端小肠中 P 物质、血清素和促胰液素免疫反应细胞之间的时间和空间相互关系。我们的研究结果证明了在向上迁移的肠内分泌细胞群体中 P 物质、血清素和促胰液素的顺序表达，此外，还鉴定了共表达 P 物质和血清素的隐窝相关细胞群体，但它们无法穿过该途径。隐窝中缺乏促胰液素免疫反应性细胞表明隐窝和/或绒毛中存在的局部因素调节促胰液素表达。 BrdU 和多标记免疫组织化学的联合使用提供了一种定义整个胃肠道肠内分泌细胞分化途径的方法。
Precise spatial interrelationships exist between substance P, serotonin, and secretin containing enteroendocrine cells in the gastrointestinal tract of mice. In the proximal small intestine these products are coexpressed in various combinations in single enteroendocrine cells along the crypt to villus axis in a pattern that suggests the sequential expression of substance P, serotonin, and secretin. In this report we use bromodeoxyuridine (BrdU) and multilabeling immunohistochemistry to define the temporal and spatial interrelationships between substance P, serotonin, and secretin immunoreactive cells in the mouse proximal small intestine. Our findings demonstrate the sequential expression of substance P, serotonin, and secretin in a population of upwardly migrating enteroendocrine cells and, furthermore, identify a population of crypt associated cells coexpressing substance P and serotonin that fails to traverse this pathway. The lack of secretin immunoreactive cells in the crypts suggests that local factors present in the crypts and/or on villi regulate secretin expression. The combined use of BrdU and multilabeling immunohistochemistry provides a method for defining enteroendocrine cell differentiation pathways throughout the gastrointestinal tract.