Bacterial Lipopolysaccharide Plus Interferon-γ Elicit a Very Fast Inhibition of a Ca2+-dependent Nitric-oxide Synthase Activity in Human Astrocytoma Cells*
Bacterial Lipopolysaccharide Plus Interferon-γ Elicit a Very Fast Inhibition of a Ca2+-dependent Nitric-oxide Synthase Activity in Human Astrocytoma Cells*
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细菌脂多糖加干扰素-γ 可快速抑制人星形细胞瘤细胞中 Ca2+ 依赖性一氧化氮合酶活性*
DOI：
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发表时间：
1997
影响因子：
4.8
通讯作者：
Giuliana Lauro
中科院分区：
文献类型：
--
作者：
M. Colasanti;E. Cavalieri;T. Persichini;V. Mollace;S. Mariotto;H. Suzuki;Giuliana Lauro
以往的研究结果表明，诱导型一氧化氮合酶(INOS)表达的诱导可能被构成一氧化氮合酶(CNOS)酶所产生的内源性NO水平所抑制。在同时拥有cNOS和iNOS的细胞类型中，这可能代表了一个明显的悖论。在此，我们报道了能够在星形细胞瘤细胞中强烈诱导诱导型一氧化氮合酶的脂多糖和干扰素-γ能够迅速抑制原生型一氧化氮合酶亚型产生的NO，从而获得诱导型一氧化氮合酶的最佳条件，并解决了明显的悖论。事实上，用脂多糖加干扰素-γ(Mix)处理T67细胞30分钟会强烈抑制cNOS的活性，这是通过测量[~3H]瓜氨酸的产生来确定的。此外，还通过测定NO的稳定分解产物亚硝酸盐来观察MIX的作用：MIX能显著减少N-甲基-D-天冬氨酸诱导的T67细胞亚硝酸盐的产生。最后，利用逆转录聚合酶链式反应，我们观察到混合处理T67细胞30分钟并不影响编码神经元型NOS-I亚型的mRNA的表达。这些结果表明，星形胶质细胞中cNOS异构体可能作为iNOS诱导的控制功能这一新概念。
Previous results indicate that induction of inducible nitric-oxide synthase (iNOS) expression may be kept suppressed by the endogenous NO level as produced by a constitutive NOS (cNOS) enzyme. In cell types possessing both cNOS and iNOS, this may represent an evident paradox. Here, we report that lipopolysaccharide and interferon-γ, which are able to strongly induce iNOS in astrocytoma cells, can rapidly inhibit the NO production generated by the constitutive NOS isoform, thus obtaining the best conditions for iNOS induction and resolving the apparent paradox. In fact, a 30-min treatment of T67 cells with the combination of lipopolysaccharide plus interferon-γ (MIX) strongly inhibits the cNOS activity, as determined by measuring [3H]citrulline production. In addition, the effect of MIX is also observed by measuring nitrite, the stable breakdown product of NO: a 30-min pretreatment of T67 cells with MIX is able to reduce significantly the N-methyl-D-aspartate-induced nitrite production. Finally, using reverse transcriptase-polymerase chain reaction, we have observed that a 30-min treatment of T67 cells with MIX does not affect expression of mRNA coding for the neuronal NOS-I isoform. These results suggest the novel concept of a possible role of a cNOS isoform in astrocytes as a control function on iNOS induction.
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DOI：
10.1073/pnas.87.2.682
发表时间：
1990-01-01
影响因子：
11.1
作者：
BREDT, DS;SNYDER, SH
通讯作者：
SNYDER, SH
DOI：
10.1016/s0006-291x(05)81351-8
发表时间：
1991-11-14
影响因子：
3.1
作者：
NAKANE, M;MITCHELL, J;MURAD, F
通讯作者：
MURAD, F
细胞因子对人类诱导型一氧化氮合酶（NOS2）基因的转录调控： 对人类 NOS2 启动子的初步分析
DOI：
10.1073/pnas.93.3.1054
发表时间：
1996-02-06
影响因子：
11.1
作者：
deVera, ME;Shapiro, RA;Geller, DA
通讯作者：
Geller, DA
影响因子：
56.9
作者：
KAMIJO, R;HARADA, H;VILCEK, J
通讯作者：
VILCEK, J
大肠杆菌内毒素抑制培养内皮细胞中激动剂介导的胞浆 Ca2 动员和一氧化氮生物合成。
影响因子：
20.1
作者：
Graier,WF;Myers,PR;Rubin,LJ;Adams,HR;Parker,JL
通讯作者：
Parker,JL