Rapid detection of measles virus in skin rashes by immunofluroescence.
Rapid detection of measles virus in skin rashes by immunofluroescence.
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通过免疫荧光快速检测皮疹中的麻疹病毒。
DOI：
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发表时间：
1976
影响因子：
6.4
通讯作者：
B. Bjorvatn
中科院分区：
文献类型：
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作者：
E. Olding;B. Bjorvatn
用特异性免疫荧光法检测皮疹中麻疹病毒抗原。使用麻疹病毒的超免疫兔和马抗血清（也与异硫氰酸荧光素结合）对皮肤穿刺活检标本的冷冻切片进行染色，作为对照。麻疹病毒在21例活检标本中的20例中得到了明确的证实，这些标本是在发病后4天内采集的。麻疹病毒抗原也被发现在三个nonexperimentatous皮肤活检标本在experimentatous阶段的前四天。病毒抗原存在于表皮、皮肤附件和真皮的单个细胞或细胞簇中。在皮疹发作后5至6天的活检标本中未检测到病毒抗原。
Specific immunofluorescence was used to detect measles virus antigen in skin rashes. Cryostat sections of punch-biopsy specimens of the skin were stained with use of hyperimmune rabbit and horse antisera to measles virus, also conjugated with fluorescein isothiocyanate, served as controls. Measles virus was specifically demonstrated in 20 of 21 biopsy specimens taken within four days after the onset of exanthema. Measles virus antigen was also found in three of five biopsy specimens from nonexanthematous skin during the first four days of the exanthematous phase. The viral antigen was found in single cells or in clusters of cells in the surface epithelium, skin appendages, and corium. No viral antigen was detected in biopsy specimens taken five to six days after the onset of rash.