Interleukin-8 and hIRH (SDF1-alpha/PBSF) mRNA expression and histological activity index in patients with chronic hepatitis C.
Interleukin-8 and hIRH (SDF1-alpha/PBSF) mRNA expression and histological activity index in patients with chronic hepatitis C.
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慢性丙型肝炎患者白细胞介素8和hIRH (SDF1-α/PBSF) mRNA表达量及组织学活性指数
DOI：
10.1002/hep.510280116
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Barnard,GF
中科院分区：
文献类型：
--
作者：
Shimoda,K;Begum,NA;Shibuta,K;Mori,M;Bonkovsky,HL;Banner,BF;Barnard,GF
重组人肝癌内分泌减少因子（hIRH）/基质细胞衍生因子1（SDF 1-α）/前B细胞生长刺激因子（PBSF）是一种新的趋化因子，在体外对中性粒细胞具有趋化作用，在体内对中性粒细胞、淋巴细胞和单核细胞具有混合趋化作用。我们研究了不同严重程度的慢性丙型肝炎中白细胞介素-8（IL-8）和hIRH的信使RNA（mRNA）表达。采用逆转录和半定量聚合酶链反应（RT-PCR）扩增方法检测37例肝活检标本中IL-8和hIRH mRNA的表达水平。我们研究了mRNA表达和组织学活动指数（HAI）的组成部分之间的相关性。HAI≥ 8的患者校正IL-8 mRNA表达率（0.24±0.13; n= 20）显著高于HAI≤ 7的患者（0.05±0.03; n= 17; P<0.01）。0001）。此外，IL-8 mRNA表达与门静脉炎症（PI）的严重程度密切相关（高PI vs.低PI，0.22±0.14 vs. 0.05±0.04; P<0.05）。0.0001）和胆管病变（0.29±0.15 vs.0.11 ±0.1; P<0.01）。01）。相反，hIRH mRNA表达与总HAI、HAI的任何组分或胆管炎症或损伤无关。这些结果表明，hIRH，虽然有-CXC-，α趋化因子基序，并表现出在体内和体外炎症活性的IL-8，在肝脏炎症和损伤中发挥不同的作用，从IL-8。IL-8表达与慢性丙型肝炎患者的炎症直接相关，而hIRH表达与炎症的组织病理学严重程度无关。
Recombinant human intercrine reduced in hepatomas (hIRH)/stromal cell-derived factor 1 (SDF1-α)/pre-B-cell growth-stimulating factor (PBSF), a new chemokine, exhibits an in vitro chemotaxis to neutrophils and a mixed in vivo chemotactic activity to neutrophils, lymphocytes, and monocytes in a rat intradermal injection model. We have investigated the messenger RNA (mRNA) expression of interleukin-8 (IL-8) and hIRH, in chronic hepatitis C of differing severity. Levels of expression of IL-8 and hIRH mRNA obtained from 37 human liver biopsy samples were measured by reverse-transcription and semiquantitative polymerase chain reaction (RT-PCR) amplification. We examined the correlation between mRNA expression and components of the histological activity index (HAI). Patients with HAI≥ 8 had a significantly higher corrected IL-8 mRNA expression ratio (0.24±0.13 [mean±SD]; n= 20) than those with HAI≤ 7 (0.05±0.03; n= 17; P<. 0001). Additionally, IL-8 mRNA expression was strongly associated with the severity of portal inflammation (PI)(high PI vs. low PI, 0.22±0.14 vs. 0.05±0.04; P<. 0001) and with the presence of bile duct lesions (0.29±0.15 vs. 0.11±0.1; P<. 01). In contrast, hIRH mRNA expression was not associated with the total HAI, any components of the HAI, or bile duct inflammation or injury. These results suggest that hIRH, although having the-CXC-, alpha chemokine motif, and exhibiting in vivo and in vitro inflammatory activity as does IL-8, plays a different role from IL-8 in hepatic inflammation and injury. IL-8 expression is directly associated with inflammation in patients with chronic hepatitis C, while hIRH expression does not correlate with histopathological severity of inflammation.