MOLECULAR-CLONING OF A NOVEL THYROID STEROID-RECEPTOR SUPERFAMILY GENE FROM CULTURED RAT NEURONAL CELLS
MOLECULAR-CLONING OF A NOVEL THYROID STEROID-RECEPTOR SUPERFAMILY GENE FROM CULTURED RAT NEURONAL CELLS
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DOI：
10.1006/bbrc.1994.2900
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发表时间：
1994-12-30
影响因子：
3.1
通讯作者：
MIKI, K
中科院分区：
文献类型：
--
作者：
OHKURA, N;HIJIKURO, M;MIKI, K
原代培养的脑神经元细胞为神经元发育（包括细胞凋亡）的分析提供了一个系统。通过PCR扩增，从核受体的保守序列设计的引物，甲状腺/类固醇受体超家族的一个新的成员被确定为NOR-1（神经元衍生的孤儿受体）的前脑神经元细胞凋亡。NOR-1的cDNA编码一个68 kDa，含628个氨基酸残基的蛋白质，其氨基酸序列与Nur 77家族在其DNA结合域高度同源，并且在其推定的配体结合域中适度同源。利用迁移率变动分析，我们发现NOR-1蛋白与B1 a反应元件结合，该元件已被鉴定为Nur 77家族的靶序列，这表明Nur 77家族的三个成员可能在不同情况下反式激活共同的靶基因。(C)1994年出版社出版。
Brain neuronal cells in primary culture provide one system for the analysis of neuronal development, including apoptosis. By PCR amplification, with primers designed from conserved sequences among nuclear receptors, a novel member of the thyroid/steroid receptor superfamily was identified from forebrain neuronal cells undergoing apoptosis and designated as NOR-1 (neuron derived orphan receptor). A cDNA done of NOR-1 encodes a 68kDa, 628 amino acid residue-containing protein, with an amino acid sequence highly homologous to the Nur77 family in its DNA-binding domain and moderately so in its putative ligand-binding domain. Using mobility shift assay, we showed that the NOR-1 protein binds to the B1a response-element which has been identified as the target sequence of the Nur77 family, suggesting that three members of the Nur77 family may transactivate common target gene(s) at different situations. (C) 1994 Academic Press, Inc.