Type II-dependent secretion of a Pseudomonas aeruginosa DING protein
Type II-dependent secretion of a Pseudomonas aeruginosa DING protein
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DOI：
10.1016/j.resmic.2012.07.007
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发表时间：
2012-07-01
影响因子：
2.6
通讯作者：
Filloux, Alain
中科院分区：
文献类型：
--
作者：
Ball, Genevieve;Viarre, Veronique;Filloux, Alain
铜绿假单胞菌是一种机会致病菌，它利用广泛的蛋白分泌系统与宿主相互作用。编码PAO 1 Hxc II型分泌系统的基因与编码磷酸酶（LapA/LapB）的基因相连。微阵列基因分型表明，铜绿假单胞菌临床分离株，包括尿路（JJ 692）和血液（X13273）分离株，缺乏lapA/lapB基因。相反，我们表明，他们携带一个基因编码的PstS家族的蛋白质。这种蛋白质，我们称之为LapC，也与LapA/LapB有显著的相似之处。LapC属于DING蛋白家族，在其N端显示典型的DINGGG基序。DING蛋白是原核生物磷酸盐结合蛋白的一种。我们发现，LapC是分泌的HXC依赖的方式，是在控制下的PhoB反应调节。在JJ 692和X13273中发现的遗传组织hxc-lapC与PA 14相似，PA 14是最常见的铜绿假单胞菌基因型。虽然LapA、LapB和LapC蛋白在铜绿假单胞菌发病机制中的作用仍不清楚，但它们可能是宿主内遇到低磷酸盐环境时生存和茁壮成长所需的磷酸盐清除或传感系统的一部分。(c)2012巴斯德研究所。由Elsevier Masson SAS出版。All rights reserved.
Pseudomonas aeruginosa is an opportunistic bacterial pathogen that uses a wide range of protein secretion systems to interact with its host. Genes encoding the PAO1 Hxc type II secretion system are linked to genes encoding phosphatases (LapA/LapB). Microarray genotyping suggested that Pseudomonas aeruginosa clinical isolates, including urinary tract (JJ692) and blood (X13273) isolates, lacked the lapA/lapB genes. Instead, we show that they carry a gene encoding a protein of the PstS family. This protein, which we call LapC, also has significant similarities with LapA/LapB. LapC belongs to the family of DING proteins and displays the canonical DINGGG motif within its N terminus. DING proteins are members of a prokaryotic phosphate binding protein superfarnily. We show that LapC is secreted in an Hxc-dependent manner and is under the control of the PhoB response regulator. The genetic organization hxc-lapC found in JJ692 and X13273 is similar to PA 14, which is the most frequent P. aeruginosa genotype. While the role of LapA, LapB and LapC proteins remains unclear in P aeruginosa pathogenesis, they are likely to be part of a phosphate scavenging or sensing system needed to survive and thrive when low phosphate environments are encountered within the host. (c) 2012 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.