Inducible nitric oxide synthase in monocytes from patients with Graves' disease.
Inducible nitric oxide synthase in monocytes from patients with Graves' disease.
复制标题
格雷夫斯病患者单核细胞中的诱导型一氧化氮合酶。
DOI：
10.1006/bbrc.1996.1420
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发表时间：
1996
影响因子：
3.1
通讯作者：
Esteban Santiago
中科院分区：
文献类型：
--
作者：
N. López;Amparo Calleja;Álvaro González;L. Pérez;M. Aymerich;M.Angeles Burrel;Esteban Santiago
诱导型一氧化氮合酶（iNOS）的存在下，新鲜的单核细胞从Graves病患者首次被证明。免疫表型分析表明，反映单核细胞的活化和分化状态的配置文件。在含有甲状腺自身抗原（TSH受体、甲状腺过氧化物酶或甲状腺球蛋白）相关序列的合成肽存在下，孵育来自健康志愿者的淋巴瘤细胞，导致单核细胞刺激，表现为表型和iNOS表达的变化。这种表达没有发生在分离的单核细胞，除非产品与Th 1活性存在于培养基中。活性肽含有已经描述的特征性“2-6-11”基序[López-Moratalla等人（1995）Biochim. Biophys. Acta 1265，181-188]。这些结果提示自身抗原在Graves病发病机制中的新作用：诱导iNOS的表达和激活单核细胞可能是自身免疫反应的基础。
The presence of inducible nitric oxide synthase (iNOS) in fresh monocytes from patients with Graves' disease was demonstrated for the first time. Immunophenotypic analysis showed a profile reflecting a state of activation and differentiation of monocytes. Incubation of lymphomononuclear cells from healthy volunteers in the presence of synthetic peptides with sequences related to thyroid autoantigens (TSH receptor, thyroid peroxidase, or thyroglobulin) led to a stimulation of monocytes manifested by a change in phenotype and expression of iNOS. This expression did not take place on isolated monocytes, unless products associated with Th1 activity were present in the medium. Active peptides contained a characteristic "2-6-11" motif already described [López-Moratalla et al. (1995) Biochim. Biophys. Acta 1265, 181-188]. These results are suggestive of a new role for autoantigens in the pathogenesis of Graves' disease: that of inducing the expression of iNOS and activating the monocyte possibly underlying the autoimmune response.