In situ Surface Infrared Study of DNA Hybridization on Au Island Films Evaporated on Silicon Surfaces
In situ Surface Infrared Study of DNA Hybridization on Au Island Films Evaporated on Silicon Surfaces
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DOI：
10.1143/jjap.48.04c186
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发表时间：
2009-04
影响因子：
1.5
通讯作者：
Kohki Tanaka;A. Hirano-Iwata;K. Miyamoto;Y. Kimura;M. Niwano
中科院分区：
文献类型：
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作者：
Kohki Tanaka;A. Hirano-Iwata;K. Miyamoto;Y. Kimura;M. Niwano
在这里，我们提出了一个无标记检测的DNA杂交的Au纳米岛薄膜沉积在硅表面的红外吸收光谱的几何形状的多次内反射（MIR-IRAS）。采用蒸发法在Si表面制备了Au纳米岛薄膜。通过Au-硫醇化学和随后的DNA杂交的DNA固定用MIR-IRAS监测。与互补DNA的相互作用在1686 cm-1处产生IRAS峰，其中出现核碱基的C=O伸缩振动模式。另一方面，添加非互补DNA在相同区域中没有引起显著的光谱变化。由于MIR几何结构与沉积在Si上的Au纳米岛膜相结合，实现了40 nM DNA的灵敏检测，该膜在C=O伸缩振动区域周围提供低背景吸收。实验结果和理论预测之间的比较，使用从头计算得到的，也进行了检查。
Here we propose a label-free detection of DNA hybridization on Au nanoisland films deposited on Si surfaces by infrared absorption spectroscopy in the geometry of multiple internal reflection (MIR-IRAS). Au nanoisland films were prepared on Si surfaces by evaporation. DNA immobilization via Au-thiol chemistry and subsequent DNA hybridization was monitored with MIR-IRAS. Interaction with complementary DNA produced an IRAS peak at ∼1686 cm-1 where the C=O stretching vibration modes of nucleobases appear. On the other hand, addition of non-complementary DNA induced no significant spectral changes in the same region. Sensitive detection of 40 nM DNA was achieved owing to the MIR geometry combined with Au nanoisland films deposited on Si which provides low-background absorption around the C=O stretching vibration region. Comparison between experimental results and theoretical prediction, obtained using the ab initio calculation, was also examined.