Mouse monoclonal IgM antibody against human lung cancer line SK-LC-3 with specificity for H(O) blood group antigen.
Mouse monoclonal IgM antibody against human lung cancer line SK-LC-3 with specificity for H(O) blood group antigen.
复制标题
针对人肺癌细胞系 SK-LC-3 的小鼠单克隆 IgM 抗体，对 H(O) 血型抗原具有特异性。
作者：
Anger,BR;Lloyd,KO;Oettgen,HF;Old,LJ
将人肺癌细胞株SK-LC-3免疫的小鼠脾细胞与小鼠骨髓瘤细胞NS-1融合。其中一个杂交克隆产生了单抗IgM抗体(命名为F-3)，用抗鼠Ig-MHA和血凝试验进行检测。该抗体可被O红细胞完全吸收，并可被低浓度的H(O)糖蛋白和猪粘蛋白(A+H)完全抑制。孟买的红细胞即使在神经氨酸酶治疗后也完全不能吸收F-3的活性。A1、A2、A1、B、A2B和B红细胞以及A-和B-糖蛋白对F-3活性的吸收或抑制作用较弱。其他糖蛋白(包括那些具有Leaor血型前体结构的糖蛋白)几乎没有或几乎没有抑制活性。携带F-3杂交瘤的裸鼠血清对O和A2红细胞的凝集效价分别为1：40,000和1：640。A1、A1B、A2B和B红细胞凝集效价为1：80或更低。因此，单抗F-3对H(O)血型决定簇具有高度的特异性。
Spleen cells from mice immunized with the human lung cancer line SK-LC-3 were fused with mouse NS-1 myeloma cells. One of the hybrid clones produced a monoclonal IgM antibody (designated F-3), detected with antimouse Ig-MHA and hemagglutination assays. This antibody was completely absorbed by O red cells and completely inhibited by low concentrations of H(O) glycoproteins and hog mucin (A + H). Bombay (Oh) red cells completely failed to absorb F-3 activity even after treatment with neuraminidase. A1, A2, A1,B, A2B, and B red cells and A- and B-glycoproteins were less effective in absorbing or inhibiting F-3 activity. Other glycoproteins (including those having Leaor blood group precursor structures) showed little or no inhibitory activity. Serum from nude mice carrying F-3 hybridoma agglutinated O and A2red cells at a titer of 1:40,000 and 1:640, respectively. A1, A1B, A2B, and B red cells were agglutinated with titers of 1:80 or less. Monoclonal antibody F-3 is, therefore, highly specific for H(O) blood group determinants.