Knockdown of OY-TES-1 by RNAi causes cell cycle arrest and migration decrease in bone marrow-derived mesenchymal stem cells
Knockdown of OY-TES-1 by RNAi causes cell cycle arrest and migration decrease in bone marrow-derived mesenchymal stem cells
复制标题
RNAi 敲低 OY-TES-1 导致骨髓间充质干细胞细胞周期停滞和迁移减少
DOI：
10.1042/cbi20120037
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发表时间：
2012-10-01
影响因子：
3.9
通讯作者：
Xie, Xiao-Xun
中科院分区：
文献类型：
--
作者：
Cen, Yan-Hui;Guo, Wen-Wen;Xie, Xiao-Xun
OY-TES-1是CTA(癌症-睾丸抗原)组的一员，在多种癌症中表达，在成人正常组织中限制性表达，但睾丸除外。为探讨骨髓间充质干细胞是否表达OY-TES-1及其在MSCs中的作用，采用逆转录聚合酶链式反应、免疫细胞化学和Western印迹方法检测了人骨髓间充质干细胞OY-TES-1的表达。利用RNAi技术下调OY-TES-1的表达，分析细胞活力、细胞周期、细胞凋亡和迁移能力。MSCs在mRNA和蛋白水平均表达OY-TES-1。这些MSCs中OY-TES-1的表达下调导致细胞生长抑制、细胞周期停滞、诱导凋亡和迁移能力减弱。这些初步结果提示，OY-TES-1可能影响MSCs的生物学行为。
OY-TES-1 is a member of the CTA (cancer-testis antigen) group expressed in a variety of cancer and restrictedly expressed in adult normal tissues, except for testis. To determine whether MSCs (mesenchymal stem cells) express OY-TES-1 and its possible roles on MSCs, OY-TES-1 expression in MSCs isolated from human bone marrow was tested with RT (reverse transcription)-PCR, immunocytochemistry and Western blot. Using RNAi (RNA interference) technology, OY-TES-1 expression was knocked down followed by analysing cell viability, cell cycle, apoptosis and migration ability. MSCs expressed OY-TES-1 at both mRNA and protein levels. The down-regulation of OY-TES-1 expression in these MSCs caused cell growth inhibition, cell cycle arrest, apoptosis induction and migration ability attenuation. Through these primary results it was suggested that OY-TES-1 may influence the biological behaviour of MSCs.