What does the eye tell the brain?: Development of a system for the large-scale recording of retinal output activity
What does the eye tell the brain?: Development of a system for the large-scale recording of retinal output activity
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DOI：
10.1109/tns.2004.832706
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发表时间：
2004-08-01
影响因子：
1.8
通讯作者：
Sher, A
中科院分区：
文献类型：
--
作者：
Litke, AM;Bezayiff, N;Sher, A
一个多电极阵列系统已经被开发出来研究视网膜如何处理和编码视觉图像。该系统可以同时记录来自数百个视网膜输出神经元的细胞外电活动，因为动态视觉图像聚焦于输入神经元。检测到的视网膜输出信号可以与视觉输入相关，以研究眼睛用来将有关视觉世界的信息发送到大脑的神经代码。该系统由以下部件组成：1）具有1.7mm敏感面积的512个平面微电极的32 × 16矩形阵列（2）;电极间距为60 μ m，电极直径为5 μ m（具有519个电极的六边形阵列正在开发中）; 2）8个64通道定制设计的集成电路，用于铂化电极和交流耦合信号; 3）8个64通道集成电路，用于放大、带通滤波和模拟多路复用信号; 4）数据采集系统; 5）数据处理软件。本文将描述该系统的设计，实验和数据分析技术，和一些第一个结果与活视网膜。该系统基于在开发用于高能物理实验的硅微带探测器过程中获得的技术和专门知识。
A multielectrode array system has been developed to study how the retina processes and encodes visual images. This system can simultaneously record the extracellular electrical activity from hundreds of retinal output neurons as a dynamic visual image is focused on the input neurons. The retinal output signals detected can be correlated with the visual input to study the neural code used by the eye to send information about the visual world to the brain. The system consists of the following components: 1) a 32 x 16 rectangular array of 512 planar microelectrodes with a sensitive area of 1.7 mm(2); the electrode spacing is 60 mum and the electrode diameter is 5 mum (hexagonal arrays with 519 electrodes are under development); 2) eight 64-channel custom-designed integrated circuits to platinize the electrodes and ac couple the signals; 3) eight 64-channel integrated circuits to amplify, band-pass filter, and analog multiplex the signals; 4) a data acquisition system; and 5) data processing software. This paper will describe the design of the system, the experimental and data analysis techniques, and some first results with live retina. The system is based on techniques and expertise acquired in the development of silicon microstrip detectors for high-energy physics experiments.