Lipase-catalyzed synthesis of capsiate analog using vanillyl alcohol and conjugated linoleic acid under vacuum system
Lipase-catalyzed synthesis of capsiate analog using vanillyl alcohol and conjugated linoleic acid under vacuum system
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DOI：
10.1016/j.procbio.2012.09.011
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发表时间：
2012-12-01
影响因子：
4.4
通讯作者：
Kim, In-Hwan
中科院分区：
文献类型：
--
作者：
Hong, Seung In;Ma, Na;Kim, In-Hwan
本文主要研究了真空系统在胶囊酸类似物合成中的应用。通过脂肪酶催化酯化，在无溶剂体系中成功合成了共轭亚油酸（CIA）的capate类似物。以香草醇和CIA为底物，以米黑根脂酶RM为生物催化剂进行酯化反应。最佳反应条件为香草醇与CIA的摩尔比为1:2，反应温度为50℃，脂肪酶量为10% （w/w，以总底物为基础）。真空的应用提高了胶囊模拟物的产率和反应速率。当真空度在66.7 ~ 1.3 kPa之间时，产率达到100 mol%。在高于13.3 kPa的真空条件下，反应仅3 h，产率达到最高。合成的胶囊模拟物中9c， 11t - cia的含量高于10t，12c-CLA。(C) 2012 Elsevier Ltd.版权所有。
This study focused on the application of vacuum system to synthesize capsiate analogs. The capsiate analogs containing conjugated linoleic acid (CIA) was successfully synthesized in solvent free system via lipase-catalyzed esterification. This esterification was carried out using vanillyl alcohol and CIA as substrates, and Lipozyme RM IM from Rhizomucor miehei as a biocatalyst. The best reaction condition was a molar ratio of 1:2 (vanillyl alcohol to CIA), a reaction temperature of 50 degrees C, and a lipase loading of 10% (w/w, based on total substrates). Application of vacuum increased the yield of capsiate analog as well as the reaction rate. When the vacuum levels were between 66.7 kPa and 1.3 kPa, an equilibrium yield of 100 mol% was achieved. The maximum yield was approached after only 3 h of reaction at the vacuum levels of higher than 13.3 kPa. The content of 9c,1 1t-CIA in capsiate analog synthesized was higher than that of 10t,12c-CLA. (C) 2012 Elsevier Ltd. All rights reserved.