Quantification of protodioscin and rutin in asparagus shoots by LC/MS and HPLC methods.
Quantification of protodioscin and rutin in asparagus shoots by LC/MS and HPLC methods.
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通过 LC/MS 和 HPLC 方法定量芦笋芽中的原薯蓣皂苷和芦丁。
DOI：
10.1021/jf0344587
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发表时间：
2003
影响因子：
6.1
通讯作者：
J. Simon
中科院分区：
文献类型：
--
作者：
Mingfu Wang;Y. Tadmor;Qingli Wu;C. Chin;S. Garrison;J. Simon
建立了芦笋中原薯蓣皂苷和芦丁的液相色谱/质谱（LC/MS）选择离子检测分析方法。芦丁和原薯蓣皂苷在芽中的分布因位置而异，最接近根茎的组织被发现是原薯蓣皂苷的丰富来源，在三个测试品系中平均水平为0.025%组织鲜重，而上部最年轻的芽组织含有最高量的芦丁，水平为0.03-0.06%组织鲜重。在分级和加工过程中被丢弃的芦笋笋的下部应该被认为是一种新的增值营养产品的有前途的来源。
A liquid chromatography/mass spectrometry (LC/MS) method with selected ion monitoring was developed and validated to analyze the contents of protodioscin and rutin in asparagus. The distribution of rutin and protodioscin within the shoots was found to vary by location, with the tissue closest to the rhizome found to be a rich source of protodioscin, at an average level of 0.025% tissue fresh weight in the three tested lines, while the upper youngest shoot tissue contained the highest amount of rutin at levels of 0.03-0.06% tissue fresh weight. The lower portions of the asparagus shoots that are discarded during grading and processing should instead be considered a promising source of a new value-added nutraceutical product.