Improved biocontrol activity of Trichoderma harzianum by over-expression of the proteinase-encoding gene prb1
Improved biocontrol activity of Trichoderma harzianum by over-expression of the proteinase-encoding gene prb1
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DOI：
10.1007/s002940050173
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发表时间：
1997-01-01
期刊：
影响因子：
2.5
通讯作者：
HerreraEstrella, A
中科院分区：
文献类型：
--
作者：
Flores, A;Chet, I;HerreraEstrella, A
利用木霉转化系统，通过增加碱性蛋白酶基因prb 1的拷贝数，提高了对真菌寄生真菌哈茨木霉的生防效果。转化体是稳定的，并携带2至10个拷贝的prb 1。在真菌-真菌互作过程中，当T.哈茨菌和立枯丝核菌在体外对峙。在液体培养中，该蛋白酶可由红球藻细胞壁诱导产生。索拉尼在温室条件下，T.将哈茨转化子接种到病原菌侵染的土壤中，显著降低了黑腐病菌引起的病害。棉花植株中的茄病
Transformation systems developed for Trichoderma spp, were utilized to improve the biocontrol efficiency of the mycoparasitic fungus Trichoderma harzianum by increasing the copy number of the basic proteinase gene prb1. The transformants were stable and carried from two to ten copies of prb1. High levels of expression of prb1 during fungus-fungus interaction were detected when T. harzianum and Rhizoctonia solani were confronted in vitro. In liquid cultures the proteinase was induced by cell walls of R. solani. Under greenhouse conditions, incorporation of T. harzianum transformants into pathogen-infested soil significantly reduced the disease caused by R. solani in cotton plants.