Effect of encapsulation or grafting on release kinetics of recombinant human bone morphogenetic protein-2 from self-assembled poly(lactide-co-glycolide ethylene oxide fumarate) nanoparticles.
Effect of encapsulation or grafting on release kinetics of recombinant human bone morphogenetic protein-2 from self-assembled poly(lactide-co-glycolide ethylene oxide fumarate) nanoparticles.
复制标题
封装或移植对自组装聚（丙交酯-共-乙交酯环氧乙烷富马酸酯）纳米粒子释放重组人骨形态发生蛋白-2动力学的影响。
DOI：
10.1002/jemt.20846
复制
发表时间：
2010
影响因子：
2.5
通讯作者：
Jabbari,Esmaiel
中科院分区：
文献类型：
--
作者：
Mercado,AngelE;Jabbari,Esmaiel
本工作的目的是比较封装在热自组装聚（丙交酯环氧乙烷富马酸酯）（PLEOF）纳米颗粒（NP）中的重组人骨形态发生蛋白-2（rhBMP-2）与接枝到琥珀酰亚胺封端的聚（丙交酯富马酸酯）（PLAF-NHS）或聚（丙交酯-共-乙交酯富马酸酯）的rhBMP-2的释放特性(PLGF-NHS) NP。两亲性 PLEOF NP 的平均尺寸为 110 ± 50 nm。疏水性 PLAF-NHS 和 PLGF-NHS NP 的平均尺寸分别为 242 ± 67 和 195 ± 42 nm。 PLEOF NPs 的 rhBMP-2 封装效率为 65% 至 93%。 rhBMP-2 与 PLAF-NHS 和 PLGF-NHS NP 的接枝效率分别为 97% ± 1% 和 98% ± 1%。 PLEOF NPs 在第一周表现出相对较高的 rhBMP-2 释放率，10 天后迅速降至零。接枝 10 和 20 μg/mL rhBMP-2 的 PLEOF NP 在降解后释放出 67% 和 80% 的活性构象蛋白质。 PLGF-NHS NP 在前 2 周内表现出 rhBMP-2 的持续释放，但在 20 天后降至几乎为零（<3 ng/天）。 PLAF-NHS NP 以两种速率显示最长的活性 rhBMP-2 持续释放时间：前 2 周的高速率为 25-35 ng/mL，随后的 2 至 6 周为 5-10 ng/mL 的低速率。 PLGF-NHS 和 PLAF-NHS NP 中分别释放出近 25% 和 50% 的 rhBMP-2，在 NP 降解后具有酶活性。 PLEOF NP 可快速释放 rhBMP-2，持续 1 周，而 PLAF-NHS NP 可缓慢释放长达 6 周。显微镜。资源。技术。 73:824–833, 2010。© 2010 Wiley-Liss, Inc.
The objective of this work was to compare the release characteristics ofRecombinant humanbone morphogenetic protein‐2 (rhBMP‐2) encapsulated in thermally self‐assembled poly(lactide ethylene oxide fumarate) (PLEOF) nanoparticles (NPs) with rhBMP‐2 grafted to succinimide‐terminated poly(lactide fumarate) (PLAF‐NHS) or poly(lactide‐co‐glycolide fumarate) (PLGF‐NHS) NPs. The amphiphilic PLEOF NPs had average size of 110 ± 50 nm. The hydrophobic PLAF‐NHS and PLGF‐NHS NPs had average size of 242 ± 67 and 195 ± 42 nm, respectively. PLEOF NPs had rhBMP‐2 encapsulation efficiency ranging from 65 to 93%. Grafting efficiency of rhBMP‐2 to PLAF‐NHS and PLGF‐NHS NPs was 97% ± 1% and 98% ± 1%, respectively. PLEOF NPs displayed a relatively high‐release rate of rhBMP‐2 in the first week, which rapidly dropped to zero after 10 days. PLEOF NPs grafted with 10 and 20 μg/mL rhBMP‐2 released 67 and 80% of the protein in the active conformation after degradation. PLGF‐NHS NPs displayed sustained release of rhBMP‐2 in the first 2 weeks but dropped to almost zero rate (<3 ng/day) after 20 days. PLAF‐NHS NPs showed the longest period of sustained release of active rhBMP‐2 at two rates: a high rate of 25–35 ng/mL in the first 2 weeks followed by a low rate of 5–10 ng/mL from 2 to 6 weeks. Nearly, 25 and 50% of the rhBMP‐2 released from PLGF‐NHS and PLAF‐NHS NPs, respectively, were enzymatically active after degradation of the NPs. PLEOF NPs provided a fast release of rhBMP‐2 for 1 week, whereas PLAF‐NHS NPs provided a slow release for up to 6 weeks. Microsc. Res. Tech. 73:824–833, 2010. © 2010 Wiley‐Liss, Inc.