RNA EDITING IN WHEAT MITOCHONDRIA PROCEEDS BY A DEAMINATION MECHANISM
RNA EDITING IN WHEAT MITOCHONDRIA PROCEEDS BY A DEAMINATION MECHANISM
复制标题
DOI：
10.1016/0014-5793(95)00991-h
复制
发表时间：
1995-10-02
期刊：
影响因子：
3.5
通讯作者：
ARAYA, A
中科院分区：
文献类型：
--
作者：
BLANC, V;LITVAK, S;ARAYA, A
种子植物的线粒体信使RNA大多数（如果不是全部的话）经历转录后修饰使用分子杂交方法，已经描述了体外RNA编辑系统，其能够忠实地再现体内观察到的小麦线粒体ATP合酶mRNA的亚基9（atp 9）的C至U变化[Araya等人，（1992）Proc，Natl，Acad，Sci，USA 89，1040-1044]。在这项工作中，我们扩展了这些研究，以更好地了解这一过程的生化机制。通过P1核酸酶消化反应产物，然后进行薄层层析来分析RNA编辑mas。用未编辑的[H-3]RNA标记在碱基上和用未编辑的[P-32]RNA标记在胞苷残基的α-磷酸上进行的实验表明，植物线粒体RNA编辑通过脱氨基机制进行。
Most if not all mitochondrial messenger RNAs from seed plants undergo a post-transcriptional modification (RNA editing) involving the conversion of some cytidine residues to uridine, Using a molecular hybridization approach, an in vitro RNA editing system, able to faithfully reproduce the in vivo observed C to U changes of subunit 9 (atp9) of wheat mitochondrial ATP synthase mRNA, has been described [Araya et al, (1992) Proc, Natl, Acad, Sci, USA 89, 1040-1044]. In this work we extend these studies to better understand the biochemical mechanism of this process. RNA editing mas analysed by P1 nuclease digestion of the reaction product followed by thin layer chromatography. Experiments performed with unedited [H-3]RNA labelled on the base and with unedited [P-32]RNA labelled at the alpha-phosphate of cytidine residues, indicate that plant mitochondrial RNA editing operates through a deamination mechanism.