Lipid Membrane-Binding Properties of Amphotericin B Deoxycholate (Fungizone) Using Surface Plasmon Resonance
Lipid Membrane-Binding Properties of Amphotericin B Deoxycholate (Fungizone) Using Surface Plasmon Resonance
复制标题
使用表面等离子共振研究两性霉素 B 脱氧胆酸盐（两性霉素B）的脂质膜结合特性
DOI：
10.2116/analsci.29.697
复制
发表时间：
2013
影响因子：
1.6
通讯作者：
H. Kamimori
中科院分区：
文献类型：
--
作者：
Masako Oka;H. Kamimori
在本研究中，我们开发了一种测定阿替霉素B（AmB）脱氧胆酸盐，Fungizone，与两个模型的脂质膜，模仿哺乳动物细胞膜和真菌膜的相互作用。我们还使用表面等离子体共振（SPR）分析了哺乳动物细胞膜和真菌膜的结合动力学。与哺乳动物细胞膜相比，真菌唑对模型真菌膜具有更高的亲和性，温度依赖性的结合选择性可以用SPR系统来表征。该方法还显示了在两种温度条件（25和37°C）下模型脂质体膜在传感器芯片上的可再现固定。在制剂与脂质膜相互作用的试验中阐明了两性霉素B药用添加剂脱氧胆酸钠的结合特性，并证实脱氧胆酸钠不影响两性霉素B的膜选择性估计。通过比较AmB与真菌唑的亲和常数，讨论了AmB与真菌唑的亲和性。结果表明，这种新建立的SPR方法可以应用于评估作为AmB的药物产品的Fungizone的脂质-膜相互作用。
In the present study, we developed an assay of interactions of amphotericin B (AmB) deoxycholate, Fungizone, with two model lipid membranes that mimicked mammal cell membranes and fungal membranes. We also analyzed the binding kinetics of the mammal cell membranes and fungal membranes using surface plasmon resonance (SPR). Fungizone showed a higher affinity for the model fungal membrane than that of the model mammal cell membrane, and the binding selectivity of Fungizone in the temperature dependence could be represented by this SPR system. This method also showed reproducible immobilization of model liposome membranes on the sensor chip under two temperature conditions (25 and 37°C). The binding characteristics of the medicinal additive of Fungizone, sodium deoxycholate, were clarified on the assay of interactions of the drug product to the lipid membrane, and it was confirmed that the sodium deoxycholate did not affect the estimation of membrane selectivity of Fungizone. Furthermore, the affinities of AmB and Fungizone were discussed by comparing these affinity constants. The results demonstrate that this newly established SPR method could be applied to estimate the lipid-membrane interaction of Fungizone as a drug product of AmB.