Frozen Red Blood Cells in Transfusion.
Frozen Red Blood Cells in Transfusion.
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DOI：
10.1016/s0377-1237(09)80057-9
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发表时间：
2009-01-01
期刊：
影响因子：
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通讯作者：
Chaudhari, C N
中科院分区：
文献类型：
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作者：
Chaudhari, C N
红细胞 (RBC) 可冷冻保存，保质期为 10 年。然而，去甘油红细胞在传统开放系统中的保质期仅为24小时，导致冷冻红细胞（FS-RBC）的使用零星。最近推出了使用 ACP 215（ACP() 215 血液细胞处理系统）的海军血液研究实验室 (NBRL) 方法，其中去甘油红细胞的保质期为 14 天。 FS-RBC单位是由单次献血制备而成，需要进行甘油化和去甘油化。描述了在 FS-RBC 中使用 ACP 215 的 NBRL 方法。去甘油单位重量在 325-350 克之间，血红蛋白为 15-18 克/分升，冻融-洗涤红细胞回收率为 87%。输注去甘油红细胞具有诸如消除紧急情况下交叉配血的需要以及减少输血反应等优点。使用 ACP 215 的 NBRL 方法进行的 FS-RBC 具有保质期长、满足大规模伤亡情况下意外的高血液需求或满足个体患者稀有血型需求等优点。 FS-RBC 可以成为印度武装部队血液计划的潜在候选者，以在和平和战争期间实现不间断的血液供应。
Red blood cells (RBCs) can be cryopreserved with shelf life of 10 years. However, shelf life of deglycerolized RBCs in conventional open system is just 24 hours, resulting in sporadic use of Frozen RBC (FS-RBC). Recently Naval Blood Research Laboratory (NBRL) method using ACP 215 (ACP() 215 Haemonetics Cell Processing System) has been introduced, where shelf life of deglycerolized RBC is 14 days. FS-RBC unit is prepared from single blood donation, which needs to be glycerolized and deglycerolized. NBRL method using ACP 215 in FS-RBC is described. Deglycerolized unit weighed between 325-350 gm with haemoglobin of 15-18 gm/dl and freeze- thaw- wash RBC recovery of 87%. Transfusion of deglycerolized RBC offered advantages such as elimination of need of crossmatching in emergent situations and reduction of transfusion reactions. FS-RBC by NBRL method using ACP 215 has advantages such as long shelf life, meeting unexpected high blood demand in mass casualties situations or availability of rare blood group requirement of individual patient. FS-RBC can be a potential candidate for Indian Armed Forces Blood programme for uninterrupted blood supply during peace and war.