Induction of ovine trophoblast cell fusion by fematrin-1 in vitro
Induction of ovine trophoblast cell fusion by fematrin-1 in vitro
复制标题
fematrin-1 体外诱导绵羊滋养层细胞融合
DOI：
10.1111/asj.12439
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发表时间：
2016
影响因子：
2
通讯作者：
Hashizume Kazuyoshi
中科院分区：
文献类型：
--
作者：
Koshi Katsuo;Nakaya Yuki;Kizaki Keiichiro;Ishiguro-Oonuma Toshina;Miyazawa Takayuki;Spencer E Thomas;Hashizume Kazuyoshi
存在于基因组中的内源性逆转录病毒在包括牛和羊在内的各种脊椎动物的胎盘形成中起着特定的作用。Fematrin‐1是在牛胎盘中发现的牛内源性逆转录病毒的包膜（Env）蛋白，参与牛胎盘中胎母杂交细胞的形成。本研究旨在阐明fematrin‐1在滋养细胞中是否具有促融合活性。另一个问题是Env蛋白是否只具有物种特异性活性。为此，我们在羊滋养细胞中转染了fematrin‐1基因，并检测了Cos‐7细胞的融合活性。虽然在中性和酸性条件下均检测到fematrin‐1的促聚变活性，但酸性条件显著增强了它。而作为阳性对照的水疱性口炎病毒G蛋白的活性较弱。然而，证实fematrin‐1与水疱性口炎病毒G蛋白融合指数的比值与先前报道相似。部分融合细胞呈多核细胞。这些结果表明，即使在不同种类的滋养层细胞中，fematrin‐1也参与了滋养层杂交细胞的形成。
Endogenous retroviruses present in the genomes take a specific role in placental formation in various vertebrates, including bovine and sheep. Fematrin‐1, which is the envelope (Env) protein of bovine endogenous retrovirus found in bovine placenta, is involved in the formation of fetomaternal hybrid cells in cattle placenta. This study was conducted to clarify whether fematrin‐1 possesses fusogenic activity in trophoblast cells. Another question is whether Env proteins only have species‐specific activity or not. For this,fematrin‐1gene was transfected in ovine trophoblast cells, and we examined fusogenic activity with Cos‐7 cells. Although fematrin‐1 fusogenic activity was detected in both neutral and acidic pH conditions, acidic condition significantly enhanced it. These activities were rather weaker than those of vesicular stomatitis virus G protein as a positive control. However, the ratio of fematrin‐1 and vesicular stomatitis virus G protein fusion index was confirmed similar to those in the previous reports. Some fusion cells showed multinucleate cells. These results imply that fematrin‐1 is involved in the formation of trophoblast hybrid cells even in different species trophoblastic cells.