Antigen 43 and type 1 fimbriae determine colony morphology of Escherichia coli K-12
Antigen 43 and type 1 fimbriae determine colony morphology of Escherichia coli K-12
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DOI：
10.1128/jb.182.4.1089-1095.2000
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发表时间：
2000-02-01
影响因子：
3.2
通讯作者：
Klemm, P
中科院分区：
文献类型：
--
作者：
Hasman, H;Schembri, MA;Klemm, P
几十年来，菌落形态一直被用作细菌学中重要的鉴定和表征标准。然而，不同的菌落类型的外观背后的分子机制却很少受到关注。O抗原的合成在大肠杆菌K-12中是不起作用的，因此菌落应该只看起来粗糙。然而，以前的报告指出，存在不同的可互换的菌落形态类型。在这项研究中，我们已经解决了两个相可变的表面结构，抗原43和1型菌毛，对菌落形态的影响。由于这些结构的差异表达，可以区分四种不同的菌落表型。flu和fim，我们得出结论，细胞上相应基因产物的存在或不存在与观察到的集落形态学形式相关。有趣的是，细菌在静态液体条件下的栖息地特异性似乎与殖民地表型相关。
Colony morphology has been used as an important identification and characterization criterion in bacteriology for many decades. However, the molecular mechanisms underlying the appearance of different colony types have been given little attention. The synthesis of O antigen is defunct in Escherichia coli K-12, and colonies should accordingly only appear to be rough. However, previous reports have noted the presence of different interchangeable colony morphology types. In this study we have addressed the influence of two phase-variable surface structures, antigen 43 and type 1 fimbriae, on colony morphology, Due to differential expression of these structures, four different colony phenotypes could be distinguished, By creating and studying defined mutants of the respective loci, i.e., flu and fim, we conclude that the presence or absence of the corresponding gene products on the cells correlates with the observed colony morphology forms. Interestingly, the habitat specificity of bacteria under static liquid conditions seems to correlate with the colony phenotypes.