Comparison of protection levels against pseudorabies virus infection of transgenic mice expressing a soluble form of porcine nectin-1/HveC and vaccinated mice
Comparison of protection levels against pseudorabies virus infection of transgenic mice expressing a soluble form of porcine nectin-1/HveC and vaccinated mice
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DOI：
10.1016/j.vetmic.2005.12.011
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发表时间：
2006-05-31
影响因子：
3.3
通讯作者：
Cherel, Pierre
中科院分区：
文献类型：
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作者：
Ono, Etsuro;Tomioka, Yukiko;Cherel, Pierre
我们最近培育出表达可溶形式猪nectin-1 (PHveCIg) 的转基因小鼠，显示出对伪狂犬病病毒 (PRV) 感染的显着抵抗力。 Nectin-1，也称为疱疹病毒进入介质 C (HveC)，是一种与病毒体糖蛋白 D (gD) 结合的 α-疱疹病毒受体。为了评价转基因小鼠表达PHveCIg诱导的PRV感染抵抗水平，直接比较了接种疫苗的小鼠和转基因小鼠的保护作用。在攻击前 4 周，通过腹腔注射 PRV 株 Begonia（一种删除了 gE 和胸苷激酶基因的减毒疫苗株），用活疫苗对小鼠进行免疫。通过鼻内途径用10LD(50)、20LD(50)或50LD(50)的PRV毒株YS-81攻击接种疫苗的转基因小鼠。在接种疫苗的小鼠中，在 20LD(50) 和 50LD(50) 的攻击中没有观察到保护作用。六只接种疫苗的小鼠中只有两只在 10LD(50) 的挑战中存活下来。相比之下，四种转基因小鼠系表现出对 PRV 感染的显着抵抗力，尽管每种病毒剂量的攻击中的存活率有所不同。这些结果清楚地证明了转基因策略在控制伪狂犬病方面的巨大潜力。 (c) 2005 Elsevier B.V. 保留所有权利。
We recently generated transgenic mice expressing a soluble form of porcine nectin-1 (PHveCIg) showing remarkable resistance to pseudorabies virus (PRV) infection. Nectin-1, also known as herpesvirus entry mediator C (HveC), is an alphaherpesvirus receptor that binds to virion glycoprotein D (gD). In order to evaluate the level of resistance to PRV infection induced by the expression of PHveCIg in the transgenic mice, the protective effects of vaccinated and transgenic mice were directly compared. Mice were immunized with a live vaccine, through intraperitoneal injection of PRV strain Begonia (an attenuated vaccine strain deleted for gE and thymidine kinase genes) at 4 weeks before challenge. The vaccinated and transgenic mice were challenged with 10LD(50), 20LD(50) or 50LD(50) of PRV strainYS-81 via intranasal route. In the vaccinated mice, no protection was observed in the challenges with 20LD(50) and 50LD(50). Only two out of six vaccinated mice survived in the challenge with 10LD(50). In contrast, four transgenic mouse lines showed significant resistance to PRV infection, although the survival rates varied in the challenge with each viral dose. These results demonstrate clearly the high potential of transgenic strategy in control of pseudorabies. (c) 2005 Elsevier B.V. All rights reserved.