AN2/NG2 protein-expressing glial progenitor cells in the murine CNS: Isolation, differentiation, and association with radial glia
AN2/NG2 protein-expressing glial progenitor cells in the murine CNS: Isolation, differentiation, and association with radial glia
复制标题
作者：
Diers-Fenger, M;Kirchhoff, F;Trotter, J
在神经发育的早期，最初的多能祖细胞的谱系特征与增殖、分化和迁移有关。少突胶质前体细胞从它们的起始处迁移到它们将髓鞘形成的轴突。我们描述了一种由少突胶质前体细胞在体外表达的细胞表面蛋白AN2，结果表明，抗AN2的抗体抑制了原代培养的少突胶质前体细胞的迁移，提示AN2抗原在其迁移中发挥了作用。最近，MALDI质谱学的结果表明，AN2是大鼠NG2蛋白的小鼠同源物。在本研究中，我们用AN2抗体分析了发育过程中和成年小鼠中枢神经系统(CNS)中AN2抗体染色的细胞，进行了NG2抗体的双重染色，并用AN2抗体从出生后早期的脑中分离出细胞，研究了细胞在体外的分化潜能。AN2和NG2抗体在中枢神经系统内显示完全重叠的细胞群。AN2/NG2表达细胞在胚胎和出生后脑中表达PDGF-α受体，而在出生后脑中表现出典型的神经胶质前体细胞的电生理特性。用AN2单抗从出生后早期脑组织分离的细胞在低血清培养中分化为少突胶质细胞，或在胎牛血清存在下分化为星形胶质细胞。在胚胎脊髓中，AN2抗体染色的细胞与放射状胶质细胞紧密相对，提示神经胶质前体细胞可能像神经元一样，以放射状胶质细胞为支架进行迁移。Glia 34：213-228,2001。(C)2001年Wiley-Liss，Inc.
During early neural development, the lineage specification of initially pluripotent progenitor cells is associated with proliferation, differentiation, and migration. Oligodendroglial progenitor cells migrate from their sites of origin to reach the axons that they will myelinate. We have described a cell-surface protein, AN2, expressed by oligodendroglial progenitor cells in vitro and showed that antibodies against AN2 inhibited the migration of cultured primary oligodendroglial progenitor cells, suggesting that the AN2 antigen plays a role in their migration. Recently, results from MALDI mass spectroscopy showed that AN2 is the mouse homologue of the rat NG2 protein. In this study, we have analyzed cells staining with AN2 antibodies during development and in the adult murine central nervous system (CNS), carried out double stainings with antibodies against NG2, and investigated the differentiation potential of cells in vitro after isolation from early postnatal brain using AN2 antibodies. AN2 and NG2 antibodies stained totally overlapping populations of cells in the CNS. AN2/NG2 expressing cells in embryonic and postnatal brain expressed the PDGF-alpha -receptor and in postnatal brain exhibited electrophysiological properties typical of glial progenitor cells. Cells isolated from early postnatal brain using AN2 monoclonal antibody developed into oligodendrocytes in low serum medium or into astrocytes in the presence of fetal calf serum. In the embryonic spinal cord, cells staining with AN2 antibodies were found closely apposed to radial glial cells, suggesting that glial precursors, like neurons, may use radial glia as scaffolds for migration. GLIA 34:213-228, 2001. (C) 2001 Wiley-Liss, Inc.