Histone replacement marks the boundaries of cis-regulatory domains
Histone replacement marks the boundaries of cis-regulatory domains
复制标题
DOI：
10.1126/science.1134004
复制
发表时间：
2007-03-09
期刊：
影响因子：
56.9
通讯作者：
Henikoff, Steven
中科院分区：
文献类型：
--
作者：
Mito, Yoshiko;Henikoff, Jorja G.;Henikoff, Steven
细胞记忆是由顺式调控元件在同源异型基因上维持的，其作用机制尚不清楚。我们通过高分辨率测量组蛋白替换水平和核小体占有率，研究了果蝇同源基因簇中的染色质。同源异型基因簇在顺式调控结构域的边界上显示出明显的组蛋白替换峰，并叠加在广泛的低替换区域上。组蛋白替换峰与核酸酶敏感部位、多梳蛋白和三胸蛋白结合部位以及核小体耗竭部位密切对应。我们的结果表明，存在一个连续的过程，破坏核小体并保持顺式调节元件的可及性。
Cellular memory is maintained at homeotic genes by cis-regulatory elements whose mechanism of action is unknown. We have examined chromatin at Drosophila homeotic gene clusters by measuring, at high resolution, levels of histone replacement and nucleosome occupancy. Homeotic gene clusters display conspicuous peaks of histone replacement at boundaries of cis-regulatory domains superimposed over broad regions of low replacement. Peaks of histone replacement closely correspond to nuclease-hypersensitive sites, binding sites for Polycomb and trithorax group proteins, and sites of nucleosome depletion. Our results suggest the existence of a continuous process that disrupts nucleosomes and maintains accessibility of cis-regulatory elements.