Differential expression of ATP-gated P2X receptors in DRG between chronic neuropathic pain and visceralgia rat models
Differential expression of ATP-gated P2X receptors in DRG between chronic neuropathic pain and visceralgia rat models
复制标题
慢性神经病理性疼痛和内脏痛大鼠模型DRG中ATP门控P2X受体的差异表达
DOI：
10.1007/s11302-015-9481-4
复制
发表时间：
2016-03-01
影响因子：
3.5
通讯作者：
Li, Chao-ying
中科院分区：
文献类型：
--
作者：
Chen, Lin;Liu, Yu-wei;Li, Chao-ying
神经病理性疼痛和内脏痛在痛觉过敏的持续时间、部位和性质方面存在差异。一般认为，伤害感受受体，包括P2 X受体，可能在伤害感受机制中发挥不同的作用。P2 X1 -7受体在神经病理性疼痛和内脏痛觉过敏中的不同作用尚未完全了解。为了探讨P2 X1 -7受体在两种痛敏模型中的表达差异，本研究采用大鼠坐骨神经慢性压迫性损伤（CCI）和新生儿结肠扩张（NCRD）模型，观察了腰骶部背根神经节（DRG）神经元的P2 X1 -7受体表达。采用免疫组织化学（IHC）和实时荧光定量聚合酶链反应（RT-PCR）技术分析P2 X1 -7受体的蛋白表达水平和核酸。我们发现，除了P2 X2和P2 X3，P2 X1和P2 X5受体的表达水平在神经病理性疼痛中增加，而P2 X4，P2 X6和P2 X7受体的表达水平在内脏痛中增加。我们的研究结果还表明，除了P2 X2/3异聚体，其他P2 X亚基也可能参与异聚体的产生，如P2 X1/5和/或P2 X2/5在神经病理性疼痛和P2 X4/6和/或P2 X4/7在内脏痛。
There are divergences between neuropathic pain and visceralgia in terms of the duration, location, and character of hyperalgesia. It is generally recognized that nociceptive receptors, including P2X receptors, may play different roles in nociceptive mechanisms. The different roles of P2X1–7 receptors have not been fully understood both in neuropathic pain and visceral hyperalgesia. In order to explore the different expressions of P2X1–7 receptors in these two hyperalgesia models, the lumbosacral dorsal root ganglion (DRG) neurons from rat sciatic nerve chronic constriction injury (CCI) model and neonatal colorectal distention (NCRD) model were studied (both the primary nociceptive neuron afferents of those two models projected to the same segment of spinal cord). Both immunohistochemistry (IHC) technique and real-time fluorescence quantitative polymerase chain reaction (RT-PCR) technology were applied to analyze the protein expression levels and nucleic acid of P2X1–7 receptors. We found that except P2X2 and P2X3, the expression levels of P2X1 and P2X5 receptors increased in neuropathic pain while those expression levels of P2X4, P2X6, and P2X7 receptors increased in visceral pain. Our results also suggested that in addition to P2X2/3 heteromeric, other P2X subunits may also involved in generation heteromeric such as P2X1/5 and/or P2X2/5 in neuropathic pain and P2X4/6 and/or P2X4/7 in visceral pain.