Multiple interleukin-2 signaling pathways differentially regulated by microgravity
Multiple interleukin-2 signaling pathways differentially regulated by microgravity
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DOI：
10.1016/s0162-3109(99)00123-x
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发表时间：
1999-11-01
期刊：
影响因子：
--
通讯作者：
Grimm, EA
中科院分区：
文献类型：
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作者：
Licato, LL;Grimm, EA
宇航员在太空飞行后已发现先天免疫缺陷。为了研究微重力对先天免疫功能的作用，我们通过使用旋转壁容器 (RWV) 培养系统，评估了在模拟微重力条件下用 IL-2 刺激的人 PBMC 的 NR 和 LAK 活性。在这些条件下，NK 和 LAK 活性的产生水平与静态烧瓶培养物中的水平相当。两种培养条件下激活的 PBMC 的表型相似，但有一个明显的例外：IL-2 受体或链 (CD25)，其在模拟微重力中未能上调。为了进一步研究 IL-2 信号传导的这种变化，我们检查了 IL-2 诱导次级细胞因子的能力。在微重力培养中，IFN γ、IL-1 β 和 TNF α 的产生几乎完全消除，这表明导致各种 IL-2 介导效应的 IL-2 信号通路在生物反应器培养条件下受到差异性调节。 (C) 1999 Elsevier Science B.V. 保留所有权利。
Defects in innate immunity have been demonstrated in astronauts after space flight. To investigate the role of microgravity on innate immune function, we evaluated NR and LAK activity of human PBMC stimulated with IL-2 under conditions of simulated microgravity, by using a rotating wall vessel (RWV) culture system. Under these conditions, both NK and LAK activity were generated at levels comparable to those found in static flask cultures. The phenotype of the activated PBMC was similar between the two culture conditions, with one notable exception: the IL-2 receptor or chain (CD25), which failed to be upregulated in simulated microgravity. To further investigate this change in IL-2 signaling, we examined the ability of IL-2 to induce secondary cytokines. The production of IFN gamma, IL-1 beta, and TNF alpha was almost completely abrogated in the microgravity cultures, suggesting that the IL-2 signaling pathways leading to various IL-2-mediated effects are differentially regulated under bioreactor culture conditions. (C) 1999 Elsevier Science B.V. All rights reserved.