Kainate receptor gene expression in the developing rat brain
Kainate receptor gene expression in the developing rat brain
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DOI：
10.1523/jneurosci.14-09-05525.1994
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发表时间：
1994-09
期刊：
影响因子：
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通讯作者：
Sabine Bahn;Benedikt Volk;William Wisdenl
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文献类型：
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作者：
Sabine Bahn;Benedikt Volk;William Wisdenl
盐盐偏好受体是嗜离子型谷氨酸受体的一个亚类，可能在大脑发育中起作用。采用原位杂交技术研究了大鼠脑发育前后5个已知基因（GluR-5、-6、-7、KA-1和KA-2）的表达情况。我们用3H- kainate放射自显影技术比较了这些基因在胚胎12天（E12）至成年发育中的联合表达模式。虽然在E12期可以检测到受体亚基（KA-1除外）的mrna，但在这一阶段没有发现3H-kainic酸结合（作为受体蛋白的指标）。然而，通过E14，在整个灰质中发现了高亲和力的盐酸盐位点，但特别是在脊髓、原始小脑和前脑腹侧结构中。所有基因的表达在胚胎晚期/产后早期达到高峰。GluR-5在开发过程中的表达表现出最有趣的特征，因为这些变化是定性的。GluR-5基因在出生前后在感觉皮层（第II、III和IV层）、中性层、中隔和丘脑的CA1海马中间神经元中表达高峰。GluR-6在新皮层扣带回出现产前表达高峰。KA-1转录本随着海马的发育而出现，并且主要局限于CA3区、齿状回和下枕等离散区域。早在12岁时就在整个中枢神经系统中发现了KA-2转录本，并一直保持不变直到成年。在E14时，GluR-5和GluR-6基因在多个外周神经节（如颅神经节、背根神经节和壁神经节）中共表达。
Kainate-preferring receptors are a subclass of ionotropic glutamate receptors that might play a role in brain development. The expression of the five known genes encoding kainate receptor subunits (GluR-5, -6, -7, KA-1, and KA-2) was studied by in situ hybridization during pre- and postnatal development of the rat brain. We compared the combined expression patterns of these genes with autoradiography using 3H- kainate in the developing brain from embryonic day 12 (E12) through to adult. Although mRNAs for the receptor subunits (except KA-1) can be detected at stage E12, 3H-kainic acid binding (as an index of receptor protein) is not found at this stage. However, by E14 high-affinity kainate sites are found throughout the gray matter, but particularly in spinal cord, primordial cerebellum, and ventral forebrain structures. All genes undergo a peak in their expression in the late embryonic/early postnatal period. GluR-5 expression during development shows the most interesting features because the changes are qualitative. The GluR-5 gene shows peaks of expression around the period of birth in the sensory cortex (layers II, III, and IV), in CA1 hippocampal interneurons in the stratum oriens, in the septum, and in the thalamus. GluR-6 shows a prenatal expression peak in the cingulate gyrus of the neocortex. KA-1 transcripts appear with the development of the hippocampus and remain largely confined to discrete areas such as the CA3 region, the dentate gyrus, and subiculum. KA-2 transcripts are found throughout the CNS from as early as E12 and remain constant until adulthood. The GluR-5 and GluR-6 genes are coexpressed in multiple peripheral ganglia (e.g., cranial nerve ganglia, dorsal root ganglia, and mural ganglia) at E14.