An antibody to the receptor for insulin-like growth factor I inhibits the growth of MCF-7 cells in tissue culture.
An antibody to the receptor for insulin-like growth factor I inhibits the growth of MCF-7 cells in tissue culture.
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DOI：
10.1016/0006-291x(87)91635-4
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发表时间：
1987-11
影响因子：
3.1
通讯作者：
Quynh T. Rohlik;David J. Adams;Frederick C. Kull;Steven Jacobs
中科院分区：
文献类型：
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作者：
Quynh T. Rohlik;David J. Adams;Frederick C. Kull;Steven Jacobs
α IR-3是一种针对胰岛素样生长因子I受体的单克隆抗体，其阻断胰岛素样生长因子I结合并抑制其活性，抑制125 I-胰岛素样生长因子I与MCF-7细胞（一种雌激素依赖性人乳腺癌细胞系）的结合，IC-50约为100 ng/ml。它还以大约相同的IC-50抑制在5%小牛血清中培养的MCF-7细胞的生长。当细胞在存在和不存在雌激素的情况下培养时都发生生长抑制，并且当细胞在低密度下生长时更明显。这些发现证明了MCF-7细胞的最佳生长需要胰岛素样生长因子I，并表明它是这些细胞中的自分泌生长因子。
Alpha IR-3, a monoclonal antibody to the insulin-like growth factor I receptor which blocks insulin-like growth factor I binding and inhibits its activity, inhibits the binding of125I-insulin-like growth factor I to MCF-7 cells (an estrogen dependent human breast carcinoma cell line) with an IC-50 of approximately 100 ng/ml. It also inhibits the growth of MCF-7 cells cultured in 5% calf serum with approximately the same IC-50. Inhibition of growth occurs both when cells are cultured in the presence and absence of estrogen and is more pronounced when cells are grown at a low density. These findings demonstrate a requirement for insulin-like growth factor I for optimal growth of MCF-7 cells and suggest that it is an autocrine growth factor in these cells.