Role of inorganic polyphosphate in promoting ribosomal, protein degradation by the ion protease in E-coli
Role of inorganic polyphosphate in promoting ribosomal, protein degradation by the ion protease in E-coli
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DOI：
10.1126/science.1061315
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发表时间：
2001-07-27
期刊：
影响因子：
56.9
通讯作者：
Kornberg, A
中科院分区：
文献类型：
--
作者：
Kuroda, A;Nomura, K;Kornberg, A
无机聚磷酸盐（polyp）是一种由数百个磷酸盐（P-i）残基组成的聚合物，在大肠杆菌中积累，以应对包括氨基酸饥饿在内的应激。在这里，我们表明，腺苷5 '-三磷酸依赖性蛋白酶Lon与息肉形成复合物，并降解大部分核糖体蛋白，包括S2，L9和L13。纯化的S2也结合到息肉，并在polyP存在下与Lon形成复合物。因此，聚P可以促进核糖体。蛋白质被Lon蛋白酶降解，从而提供响应饥饿所需的氨基酸。
Inorganic polyphosphate (polyp), a polymer of hundreds of phosphate (P-i) residues, accumulates in Escherichia coli in response to stresses, including amino acid starvation. Here we show that the adenosine 5'-triphosphate-dependent protease Lon formed a complex with polyp and degraded most of the ribosomal proteins, including S2, L9, and L13. Purified S2 also bound to polyp and formed a complex with Lon in the presence of polyP. Thus, polyP may promote ribosomal. protein degradation by the Lon protease, thereby supplying the amino acids needed to respond to starvation.