In vivo dark-field reflection-mode photoacoustic microscopy
In vivo dark-field reflection-mode photoacoustic microscopy
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DOI：
10.1364/ol.30.6.000625
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发表时间：
2005-03-15
期刊：
影响因子：
3.6
通讯作者：
Wang, LHV
中科院分区：
文献类型：
--
作者：
Maslov, K;Stoica, G;Wang, LHV
设计并实现了反射式光声显微术，采用暗场激光脉冲照明和高数值孔径超声检测，对体内皮肤血管进行非侵入性成像。暗场光学照明最小化由来自表面结构的强光声信号引起的干扰。高数值孔径声透镜提供高横向分辨率，在该系统中为45-120 μ m。宽带超声波检测系统提供高轴向分辨率，估计接近15 μ m。光学照明和超声波检测采用同轴共焦配置，以获得最佳图像质量。该系统能够在生物组织中成像深达3 mm的光吸收对比度。(C)2005年，美国光学学会。
Reflection-mode photoacoustic microscopy with dark-field laser pulse illumination and high-numerical-aperture ultrasonic detection is designed and implemented in noninvasively imaged blood vessels in the skin in viuo. Dark-field optical illumination minimizes the interference caused by strong photoacoustic signals from superficial structures. A high-numerical-aperture acoustic lens provides high lateral resolution, 45-120 mum in this system. A broadband ultrasonic detection system provides high axial resolution, estimated to be similar to15 mum. The optical illumination and ultrasonic detection are in a coaxial confocal configuration for optimal image quality. The system is capable of imaging optical-absorption contrast as deep as 3 mm in biological tissue. (C) 2005 Optical Society of America.