NATURE OF POLYPEPTIDE ENCODED BY EACH OF 10 DOUBLE-STRANDED-RNA SEGMENTS OF REOVIRUS TYPE-3
NATURE OF POLYPEPTIDE ENCODED BY EACH OF 10 DOUBLE-STRANDED-RNA SEGMENTS OF REOVIRUS TYPE-3
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DOI：
10.1016/0042-6822(78)90199-x
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发表时间：
1978-01-01
期刊：
影响因子：
3.7
通讯作者：
JOKLIK, WK
中科院分区：
文献类型：
--
作者：
MCCRAE, MA;JOKLIK, WK
在适当的变性条件下，呼肠孤病毒的双链RNA片段可以在无细胞蛋白质合成系统中翻译。由于呼肠孤病毒RNA的所有10个片段都可以以几乎纯的形式分离出来，这为确定每个片段编码的多肽的性质提供了一种方法。确定了3型呼肠孤病毒Dearing株的完整编码分配集。多肽鉴定是根据磷酸基和三甘氨酸（Laemmli）基聚丙烯酰胺凝胶体系的电泳迁移率，以及葡萄球菌V8蛋白酶消化后体外翻译产物和真正的肠孤病毒多肽的肽谱进行的。后一种方法提供了绝对的识别。分配集是（使用普遍接受的ds RNA片段名称，但新提出的多肽命名法）：片段L1编码次要病毒粒子成分。lambda。3，片段L2和L3为2个主要病毒粒子核心组件。2和。lambda。1、分别;片段M1编码一个次要病毒粒子成分。mu。2、M2片段编码以小组分形式存在于病毒粒子中的多肽。作为主成分。mu。1C由其裂解而来，M3段编码非结构多肽。片段S1编码次要的外衣壳组分。sigma。1、段S2代码为核心元件。sigma。2、片段S3编码非结构多肽。sigma。NS和S4段为主要的外衣壳成分。
Under suitable conditions of denaturation, the double-stranded (ds) RNA segments of reovirus can be translated in cell-free protein synthesizing systems. Since all 10 segments of reovirus ds RNA can be isolated in virtually pure form, this provides a means for determining the nature of the polypeptide encoded by each individual segment. The complete coding assignment set was determined for the Dearing strain of reovirus serotype 3. Polypeptide identification was made on the basis of electrophoretic migration rates in the phosphate- and Tri-glycine (Laemmli)-based polyacrylamide gel systems, and on the basis of comparing peptide profiles of in vitro translation products and authentic reovirus polypeptides after digestion with staphylococcal V8 protease. The latter method provides absolute identification. The assignment set is (using the commonly accepted designation for the ds RNA segments, but a newly proposed nomenclature for the polypeptides): segment L1 codes for the minor virion component .lambda.3, and segments L2 and L3 code for the 2 major virion core components .lambda.2 and .lambda.1, respectively; segment M1 codes for a minor virion component .mu.2, segment M2 codes for the polypeptide that is present in virions in the form of the minor component .mu.1 and as the major component .mu.1C which is derived from it by cleavage and segment M3 codes for the nonstructural polypeptide .mu.NS; and segment S1 codes for the minor outer capsid shell component .sigma.1, segment S2 codes for the core component .sigma.2, segment S3 codes for the nonstructural polypeptide .sigma.NS and segment S4 codes for the major outer capsid shell component .sigma.3.