Flexible Organic Electrochemical Transistors for Highly Selective Enzyme Biosensors and Used for Saliva Testing
Flexible Organic Electrochemical Transistors for Highly Selective Enzyme Biosensors and Used for Saliva Testing
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DOI：
10.1002/adma.201404378
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发表时间：
2015-01-27
影响因子：
29.4
通讯作者：
Yan, Feng
中科院分区：
文献类型：
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作者：
Liao, Caizhi;Mak, Chunhin;Yan, Feng
DOI：10.1002/ADMA。201404378根据基质的不同，范围从1×10−7到1×10−3 M。[27]人体内UA水平异常是许多疾病的征兆，包括痛风、高尿酸血症、莱施尼汉综合征、肾脏疾病，甚至心血管疾病。因此，快速准确地测定UA对这些疾病的诊断和治疗至关重要。然而，传统的UA测量技术通常存在劳动强度大、成本高、耗时长、不灵敏和不可移植等缺点。[31-37]在这里，我们首次研究了基于OECT的UA传感器，其设备结构如图1a所示。UA传感器具有良好的选择性，检测下限为≈1×10−8M，比使用相同酶电极的常规电化学方法高出近3个数量级。其他高性能的酶生物传感器，包括胆固醇和葡萄糖传感器，也采用了同样的策略。最后，将该装置用于唾液检测，可以选择性地测量唾液中尿酸和葡萄糖的浓度。在厚度约为50微米的聚对苯二甲酸乙二醇酯(PET)衬底上制备了含铂电极和聚(3，4-乙二氧基噻吩基)：聚(苯乙烯磺酸)(PEDOT：PSS)有源层的柔性电极。柔性电极可以附着在各种可变形的表面上，并在高达1000次的弯曲试验中表现出稳定的性能，如图2a，b所示。由于栅极上的下列反应，带有铂电极的柔性电极对H_2O_2敏感：[27]
DOI: 10.1002/adma. 201404378 across a wide range from 1× 10− 7 to 1× 10− 3 M, depending on the matrix.[27] Abnormal UA level in human body is a sign for many diseases, including gout, hyperuricemia, Lesch–Nyhan syndrome, renal diseases, and even cardiovascular disorder.[28–30] Consequently, fast and accurate determination of UA is critical to the diagnosis and treatment of these diseases. However, the conventional techniques for UA measurements usually suffer from several drawbacks like labor-intensive, expensive, time-consuming, insensitive, and unportable.[31–37] Here, we studied OECT-based UA sensors with the device structure shown in Figure 1a for the first time. The UA sensors demonstrated good selectivity and a detection limit of≈ 1× 10− 8 M, which is approximately three orders of magnitudes better than conventional electrochemical methods using the same enzyme electrodes. Other high-performance enzyme biosensors, including cholesterol and glucose sensors, were realized with the same strategy. In the end, the devices were demonstrated for saliva testing and the concentrations of UA and glucose in saliva can be selectively measured. Flexible OECTs with Pt electrodes and poly (3, 4-ethylene dioxythiophene): poly (styrene sulfonate)(PEDOT: PSS)) active layers were fabricated on thin polyethylene terephthalate (PET) substrates with thicknesses of about 50 µm. The flexible OECTs can be attached on various deformable surfaces and show stable performance during bending tests up to 1000 times, as shown in Figure 2a, b. The OECTs with Pt gate electrodes are sensitive to H2o 2 due to the following reaction on the gate:[27]