A quantitative method for assessing stages of the rat estrous cycle
A quantitative method for assessing stages of the rat estrous cycle
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DOI：
10.1080/10520290500138422
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发表时间：
2005-03-01
影响因子：
1.6
通讯作者：
Johnson, JR
中科院分区：
文献类型：
--
作者：
Hubscher, CH;Brooks, DL;Johnson, JR
性别和/或激素变化对各种神经功能的影响使得在研究特定物种的雄性或雌性（或两者）之间做出选择变得困难。虽然雌性大鼠被广泛用于实验，但很少有研究控制发情期。关于如何区分发情周期的各个阶段的更详细的信息是必要的。对于本研究，每天获得一次阴道涂片，并使用巴氏涂片（PAP）程序进行染色。提供了未染色的“湿”样品和相应的PAP染色涂片的图像，其说明了周期的每个阶段以及卵巢切除术后的细胞概况。对整个周期中的不同细胞群进行定量，并确定比率，以显示发情周期每个阶段中主要细胞类型和其他细胞类型之间的趋势。染色和未染色的图像和细胞定量数据为区分发情周期的阶段提供了有价值的指导方针。
The impact of gender and/or hormone variations on a wide variety of neural functions makes the choice between studying males or females (or both) of a given species difficult. Although female rats are widely used experimentally, few studies control for the stage of estrus. More detailed information about how to distinguish the various stages of the estrous cycle is needed. For the present study, vaginal smears were obtained once a day and stained using an adaptation of the Papanicolaou (PAP) procedure. Images are provided of unstained "wet" samples and the corresponding PAP stained smears illustrating the cellular profile for each stage of the cycle as well as post-ovariectomy. The different cell populations across the cycle were quantified and ratios determined to show trends between the predominant and other cell types in each stage of the estrous cycle. Both stained and unstained images and cell quantification data provide valuable guidelines for distinguishing the stages of the estrous cycle.