Synthesis of Polysaccharides in Phragmoplasts Isolated from Tobacco BY-2 Cells
Synthesis of Polysaccharides in Phragmoplasts Isolated from Tobacco BY-2 Cells
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烟草 BY-2 细胞成膜体中多糖的合成
DOI：
10.1093/oxfordjournals.pcp.a078262
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发表时间：
1992
影响因子：
4.9
通讯作者：
H. Shibaoka
中科院分区：
文献类型：
--
作者：
T. Kakimoto;H. Shibaoka
从烟草培养细胞中获得的离体片质体制备物的放射自显影实验表明，与UDP-[3H]葡萄糖不溶性物质结合的放射性仅存在于离体片质体的细胞板上。从UDP-[14C]葡萄糖分离的片质体中，大部分放射性被1,3-β-葡聚糖酶溶解，且溶解后的放射性仅与葡萄糖相关，说明大部分放射性被1,3-β-葡聚糖溶解。在存在高浓度未标记的UDP-葡萄糖的情况下，分离的片质体含有来自UDP-[3H]木糖的放射性。不溶性物质中的大部分放射性存在于分布在细胞核周围的几个位置，而在细胞板上发现的很少。
Autoradiographic experiments using preparations of isolated phragmoplast obtained from tobacco cultured cells revealed that the radioactivity incorporated into insoluble material from UDP-[3H]glucose was exclusively present at the cell plate of isolated phragmoplasts. Most of the radioactivity incorporated into isolated phragmoplasts from UDP-[14C]glucose was solubilized by 1,3-β-glucanase and the solubilized radioactivity was associated only with glucose, indicating that most of the radioactivity was incorporated into 1,3-β-glucan. In the presence of high concentrations of unlabeled UDP-glucose, isolated phragmoplasts incorporated radioactivity from UDP-[3H]xylose. Most of the radioactivity incorporated into insoluble material was present at several sites distributed around the nuclei, while only little was found at the cell plate.