Soil microbial community variation with time and soil depth in Eurasian Steppe (Inner Mongolia, China)
Soil microbial community variation with time and soil depth in Eurasian Steppe (Inner Mongolia, China)
复制标题
欧亚草原土壤微生物群落随时间和土壤深度的变化（中国内蒙古）
DOI：
10.1186/s13213-021-01633-9
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发表时间：
2021-05
影响因子：
3
通讯作者：
Fan Yueyue
中科院分区：
文献类型：
--
作者：
Zhao Hongbin;Zheng Wenling;Zhang Shengwei;Gao Wenlong;Fan Yueyue
土壤微生物在草地生态系统物质和能量循环中起着不可或缺的作用。这些生物的丰度因环境因素而异，如一年中的时间和土壤深度。针对这些时空变化对退化典型草原土壤微生物群落变化的研究较少。方法2018年5 - 9月，在锡林郭勒典型退化草原区采集不同深度（10、20和30 cm）土壤样品90个，分析土壤细菌和真菌的群落结构和多样性，以及这些时空变化因素对群落结构的影响。利用高通量测序技术对土壤理化性质和土壤微生物多样性进行了研究。10 cm处的细菌和真菌多样性高于20 cm和30 cm处。酸细菌、变形菌、放线菌、子囊菌和担子菌的丰度随深度变化很大。土壤pH值随深度的增加而显著升高，土壤有机质（SOM）、速效氮（AN）、土壤体积含水量（VWC）和土壤温度（ST）随深度的增加而显著降低。最后，土壤深度、总有机碳（TOC）和总氮（AN）对土壤微生物和真菌群落丰度有显著影响（p< 0.05）。土壤有机质、VWC、变形菌和放线菌的丰度与土壤深度呈正相关，而pH、酸杆菌、子囊菌和担子菌的丰度与土壤深度呈负相关。我们推测，SOM和VWC占的变化，丰富的酸细菌和变形菌，而pH值的变化，放线菌，子囊菌和担子菌的丰度。
PurposeSoil microorganisms play an indispensable role in the material and energy cycle of grassland ecosystems. The abundance of these organisms vary according to environmental factors, such as time of year and soil depth. There have been few studies on the transformation of soil microbial communities in degraded typical steppe according to these temporal and spatial changes. In this study, we analyze the community structure and diversity of soil bacteria and fungi, and the impact of these changing temporal and spatial factors upon the community structure.MethodsFrom May to September 2018, we collected 90 soil samples from different depths (10, 20, and 30 cm) from the typical degraded steppe area of Xilingol. We carried out studies on soil physical and chemical properties and soil microbial diversity using high-throughput sequencing technology.ResultsWe found that depth significantly affected abundance and diversity of bacteria and fungi. Bacteria and fungi diversity at 10 cm was higher than that at 20 cm and 30 cm. The abundance of Acidobacteria, Proteobacteria, Actinomycetes, Ascomycetes, and Basidiomycetes varies significantly with depth. In addition, soil pH increased significantly with increasing depth, while soil organic matter (SOM), available nitrogen (AN), volume water content of soil (VWC), and soil temperature (ST) decreased significantly with increasing depth. Finally, the depth, total organic carbon (TOC), and AN had a significant impact on the bacterial and fungal communities’ abundance (p< 0.05).ConclusionsSpatial heterogeneity (in soil depth) is more significant than the time of year (month) in predicting changes in microbial community composition and soil properties. SOM, VWC, and the abundance of Proteobacteria and Actinomycetes positively correlate with soil depth, while pH and the abundance of Acidobacteria, Ascomycetes, and Basidiomycetes negatively correlate with soil depth. We speculate that SOM and VWC account for the variations in the abundance of Acidobacteria and Proteobacteria, while pH causes variations in the abundance of Actinomycetes, Ascomycetes and Basidiomycota.