Direct ultrastructural imaging of macrophages using a novel x-ray contact microscopy.
Direct ultrastructural imaging of macrophages using a novel x-ray contact microscopy.
复制标题
使用新型 X 射线接触显微镜对巨噬细胞进行直接超微结构成像。
DOI：
10.1046/j.1525-1373.1999.d01-4.x
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Yamamoto,Y
中科院分区：
文献类型：
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作者：
Kado,M;Richardson,M;Rajyaguru,JM;Muszynski,MJ;Friedman,H;Yamamoto,Y
一种使用台式 Nd:玻璃激光系统的紧凑、高分辨率、激光等离子体、X 射线接触显微镜方法已被开发出来。该X射线显微镜系统用于观察巨噬细胞超微结构。这些图像是使用邻近成像技术生成的，其中由激光等离子体产生的水窗 (23Å–44Å) 附近和内部波长的 5 ns 软 X 射线脉冲被样本吸收，然后记录在光刻胶上。然后将印在光刻胶上的 X 射线图像显影并用原子力显微镜 (AFM) 进行分析。通过这种新型 X 射线显微镜检查悬浮液中由硫代乙醇酸引发的小鼠腹膜巨噬细胞。将巨噬细胞的 X 射线图像与传统透射电子显微镜 (TEM) 观察到的图像进行比较。 X射线图像没有显示明显的细胞器，包括细胞核和内质网，如TEM所示，但观察到由碳的质量分布引起的高对比度和低对比度结构。因此，使用 X 射线显微镜，我们观察到了巨噬细胞超微结构的第一张 X 射线图像。巨噬细胞超微结构的成功 X 射线成像表明，邻近 X 射线显微镜对于研究与细胞动力学相关的生理学可能具有价值。
A compact, high‐resolution, laser‐plasma, x‐ray contact microscopy method using a table‐top Nd:glass laser system has been developed. This x‐ray microscopy system was applied for the observation of macrophage ultrastructures. These images were produced using proximity imaging in which a 5‐ns pulse of soft x‐rays with wavelengths near and inside the water windows (23Å–44Å) produced by the laser‐plasma were absorbed by the specimen and then registered on a photo resist. The x‐ray images imprinted on the photo resist were then developed and analyzed with an atomic force microscope (AFM). Mouse thioglycollate‐elicited peritoneal macrophages in suspension were examined by this new x‐ray microscope. The x‐ray images of the macrophages were compared with those observed by conventional transmission electron microscopy (TEM). The x‐ray images showed no obvious organelles, including the nucleus and endoplasmic reticulum, as can be seen with TEM, but high‐ and low‐contrast structures caused by mass distribution of carbon were observed. Thus, using the x‐ray microscopy we visualized the first x‐ray images of macrophage ultrastructures. The successful x‐ray imaging of macrophage ultrastructure indicates that proximity x‐ray microscopy may be of value in studying physiology linked to the dynamics of a cell.