Chemical composition and biological activity of the essential oil obtained from Bupleurum marginatum (Apiaceae)
Chemical composition and biological activity of the essential oil obtained from Bupleurum marginatum (Apiaceae)
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DOI：
10.1211/jpp/61.08.0012
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发表时间：
2009-08-01
影响因子：
3.3
通讯作者：
Wink, Michael
中科院分区：
文献类型：
--
作者：
Ashour, Mohamed L.;El-Readi, Mahmoud;Wink, Michael
目的边叶柴胡（Bupleurummarginatum）是我国南方和西南地区特有的一种草本植物。它被广泛用于许多中国处方。目的：研究水提B挥发油的化学成分、抗氧化、抗炎、抑菌和体外细胞毒活性。方法采用挥发油提取法对B.采用毛细管气相色谱（GLC/FID）和气相色谱-质谱（GLC/MS）联用技术对马边草进行了分析。采用还原二苯苦肼（DPPH中心点）和防止脱氧核糖降解的能力来评价油的抗氧化活性。用前列腺素E-2和脂氧合酶的抑制来评估抗炎活性。在体外研究了对一系列细菌和真菌的抗微生物活性。采用MTT法检测了精油对6种人癌细胞株（HepG 2、Caco-2、CCRF-CEM、HeLa、MiaPaCa-2和MCF-7）的体外细胞毒性。主要成分为十三烷（13.18%）、十一烷（10.42%）、十五烷（8.71%）、β-亚麻烯（5.53%）和β-亚麻烯氧化物（5.29%）。油还原二苯基苦肼（DPPH中心点）和防止脱氧核糖降解的能力用于评价抗氧化活性，发现相应的IC 50值（导致活性抑制降低50%的药物浓度）分别为3.66 mg/ml和17.4 μ g/ml。对前列腺素E-2产生和脂氧合酶的抑制被用于评估抗炎活性（对于脂氧合酶的IC 50为63.64 μ g/ml，在25 μ g/ml剂量下对前列腺素E-2的抑制为26.04%）。该油还显示出对革兰氏阳性病原体（化脓性链球菌和无乳链球菌）的显著体外抗菌活性，最小抑菌浓度（MIC）值范围为0.125至4.00 mg/ml。用MTT法测定了该精油对6种人癌细胞系（HepG 2、Caco-2、CCRF-CEM、HeLa、MiaPaCa-2和MCF-7）的体外细胞毒性，结果显示，在CCRF-CEM细胞系中活性最高，处理24 h后的IC 50（导致细胞活力降低50%的浓度）为46.01 μ g/ml。marginatum具有良好的抗炎活性，沿着对多种肿瘤细胞（CCRF-CEM和HepG 2）的细胞毒作用，并通过诱导细胞凋亡而发挥作用，这一体外活性使其在局部的传统应用成为可能。然而，其有限的抗菌活性表明，与其他药物的组合是有效使用的必要条件。需要进一步的选择性测试来评估油对正常细胞的影响。
Objectives Bupleurum marginatum is a herb indigenous to the southern and southwestern part of China. It is widely used in many Chinese prescriptions. We aimed to investigate the chemical composition, antioxidant, anti-inflammatory, antimicrobial and in-vitro cytotoxic activity of the hydrodistilled and extracted essential oil from B. marginatum to validate some of its ethnopharmacologial uses.Methods The essential oil of the aerial parts of B. marginatum was analysed by capillary gas chromatography (GLC/FID) and gas chromatography-mass spectrometry (GLC/MS). The ability of the oil to reduce diphenylpicrylhydrazine (DPPH center dot) and to prevent the degradation of deoxyribose were used to evaluate the antioxidant activity. Inhibition of both prostaglandin E-2 production and lipoxygenase were used to assess the anti-inflammatory activity. Antimicrobial activity was studied in vitro against a range of bacteria and fungi. The in-vitro cytotoxicity of the essential oil on six human cancer cell lines (HepG2, Caco-2, CCRF-CEM, HeLa, MiaPaCa-2 and MCF-7) was examined using the MTT assay.Key findings Seventy-two components, comprising almost 94.29% of the total peak area, were identified in the analysis. The main components were tridecane (13.18%), undecane (10.42%), pentadecane (8.71%), beta-caryophyllene (5.53%) and beta-caryophyllene oxide (5.29%). The ability of the oil to reduce diphenylpicrylhydrazine (DPPH center dot) and to prevent the degradation of deoxyribose were used to evaluate the antioxidant activity and the corresponding IC50 values (drug concentration which resulted in a 50% reduction in inhibition of the activity) were found to be 3.66 mg/ml and 17.4 mu g/ml, respectively. Inhibition of both prostaglandin E-2 production and lipoxygenase were used to assess the anti-inflammatory activity (IC50 of 63.64 mu g/ml for lipoxygenase, 26.04% inhibition of prostaglandin E-2 at 25 mu g/ml dose). The oil also showed a significant in-vitro antimicrobial activity against Gram positive pathogens (Streptococcus pyogenes and Streptococcus agalactiae) with minimum inhibitory concentration (MIC) values ranging from 0.125 up to 4.00 mg/ml. The in-vitro cytotoxicity of the essential oil on six human cancer cell lines (HepG2, Caco-2, CCRF-CEM, HeLa, MiaPaCa-2 and MCF-7) examined using the MTT assay revealed the highest activity to be in the CCRF-CEM cell line with an IC50 (concentration which resulted in a 50% reduction in cell viability) of 46.01 mu g/ml after 24 h treatment.Conclusions The essential oil of B. marginatum exhibited a promising anti-inflammatory activity along with strong cytotoxicity against many cancer cells (CCRF-CEM and HepG2) mediated through induction of apoptosis, and this in-vitro activity make its local traditional uses rational. However, its limited antimicrobial activity indicates that a combination with other drugs is essential for effective use. Further selectivity testing is required to evaluate the effect of the oil against normal cells.