Effects of exogenous LH or FSH on endogenous FSH, progesterone and estradiol secretion.
Effects of exogenous LH or FSH on endogenous FSH, progesterone and estradiol secretion.
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外源性 LH 或 FSH 对内源性 FSH、孕酮和雌二醇分泌的影响。
DOI：
10.1095/biolreprod18.5.820
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发表时间：
1978
影响因子：
3.6
通讯作者：
W. Talley
中科院分区：
文献类型：
--
作者：
N. Schwartz;W. Talley
在一组实验中，在发情前期的下午，向接受戊巴比妥（PB）的大鼠注射排卵剂量的绵羊LH或FSH。在1000 h发情期，血清FSH高于未注射对照组。肾上腺对这种反应不是必需的。在促性腺激素注射前不久进行卵巢切除术，导致第二天早上血清FSH升高，注射LH或FSH后血清FSH未进一步升高。在任何处理组中，在1000 h发情时血清LH均未升高，垂体LH含量也未降低。在第二组实验中，在3个处理组中研究了发情前期下午（1630 h）和发情期早晨（1000 h）之间LU、FSH、雌二醇（E2）和孕酮（P4）的时间过程：a）生理盐水对照（无PB），B）PB对照（无LH），c）PB + LH（1530 h时8 Mg IV）。a）生理盐水对照组在1830 h时显示最大LU和FSU初始峰; LH在2300 h时恢复至基线，而FSH在夜间缓慢下降。P4在1830和2300 h升高，然后下降至基线; E2在1630和1830 h之间突然下降。B）在PB对照组中，血清LU和P持续较低，但FSH在2300 h和0400 h（发情）出现小幅升高。E2也显著下降，但没有生理盐水对照组低。c）LH与PB叠加，在1630 h时升高LH，但没有进一步升高，而FSH逐渐升高，在0400 h达到峰值，然后到1000 h时部分降至盐水对照水平。因此，FSH的释放似乎与LU无关。注射LU还诱导P4在1630 h和1830 h升高，E2在1630 h和1830 h之间下降，与盐水对照相同。在第三个实验中，测试P4注射，叠加PB阻断。P4未能增加LH或FSH（以类似于LH注射的方式），但确实导致E2下降。它的结论是，主要LH（和/或FSH）浪涌，以及外源性LH或FSH叠加PB封锁，可以诱导FSH的释放独立的测量类固醇（血清P4，E2）的变化，并在没有伴随LH释放。在没有卵巢的情况下不能检测到FSH释放的诱导，但卵巢切除术本身增加了FSH释放而没有LU释放。这些数据表明，促性腺激素，以及卵巢切除术，可能会通过改变卵巢的非甾体反馈诱导FSH的释放。
In one set of experiments, an ovulatory dose of ovine LH or FSH was injected, on the afternoon of proestrus, into rats which had received pentobarbital (PB). Serum FSH was elevated over noninjected controls at 1000 h estrus. The adrenal glands were not necessary for this response. Ovariectomy, performed shortly before the gonadotrophin injection, caused an elevation in serum FSH the following morning, which was not further increased by the injection of LH or FSH. Serum LH was not elevated nor was pituitary LH content decreased at 1000 h estrus in any treatment group. In a second set of experiments, the time course of LU, FSH, estradiol (E2) and progesterone (P4) was investigated, between proestrous afternoon (1630 h) and estrous morning (1000 h), in 3 treatment groups: a) saline controls (no PB), b) PB controls (no LH), c) PB + LH (8 Mg IV at 1530 h). a) Saline controls showed a maximum LU and FSU primary surge at 1830 h; LH returned to baseline by 2300 h, while FSH fell slowly through the night. P4 was elevated at 1830 hand 2300 h, then fell to baseline; E2 fell abruptly between 1630 h and 1830 h. b) In PB controls, serum LU and P were continuously low, but a small rise in FSH occurred at 2300 h and 0400 h (estrus). E2 fell significantly also, but not as low as in the saline controls. c) LH, superimposed on PB, raised LH at 1630 h, but no further rises occurred, while FSH rose gradually to peak at 0400 h and then fell part way to saline control levels by 1000 h estrus. Thus, FSH appeared to be released independently of LU. The injected LU also induced a rise in P4 at 1630 h and 1830 h, with E2 falling, as in saline controls, between 1630 h and 1830 h. In a third experiment, P4 injection was tested, superimposed on PB blockade. P4 failed to increase LH or FSH (in a manner resembling LH injection), but did cause E2 to fall. It is concluded that the primary LH (and/or FSH) surge, as well as exogenous LH or FSH superimposed on PB blockade, can induce a release of FSH independently of changes in measured steroids (serum P4, E2) and in the absence of concomitant LH release. The induction of FSH release could not be detected in the absence of the ovary, but ovariectomy by itself increased FSH release without LU release. The data suggest that gonadotrophins, as well as ovariectomy, may induce FSH release by altering a nonsteroidal feedback from the ovaries.