Severity of controlled cortical impact traumatic brain injury in rats and mice dictates degree of behavioral deficits
Severity of controlled cortical impact traumatic brain injury in rats and mice dictates degree of behavioral deficits
复制标题
DOI：
10.1016/j.brainres.2009.06.067
复制
发表时间：
2009-09-01
期刊：
影响因子：
2.9
通讯作者：
Borlongan, Cesar V.
中科院分区：
文献类型：
--
作者：
Yu, Seongjin;Kaneko, Yuji;Borlongan, Cesar V.
创伤性脑损伤（TBI）的临床表现包括轻度、中度或重度头部损伤，导致长期甚至永久性残疾。在动物模型中重现这种临床情况，应该可以检查创伤的病理生理学及其治疗。迄今为止，只有少数研究证明了TBI动物模型涵盖了三个创伤严重程度水平。因此，在本研究中，我们的特点是在小鼠和大鼠的脑组织病理学和行为的变化，在一系列的损伤程度所产生的轻度，中度和重度TBI的控制皮质撞击损伤技术。在这里，我们复制了先前观察到的TBI严重程度依赖性脑损伤，如在大鼠中通过2，3，5-三苯基四唑氯化物染色（严重>中度>轻度）所揭示的，但也扩展了小鼠中的这种组织病理学变化模式。此外，我们发现TBI暴露的大鼠和小鼠的运动和认知行为严重依赖性异常。总之，这些结果支持使用啮齿动物模型TBI作为一个敏感的平台，调查损伤引起的神经结构和行为缺陷，这应该作为测试实验治疗的关键结果参数。(C)2009爱思唯尔有限公司版权所有。
The clinical presentation of traumatic brain injury (TBI) involves either mild, moderate, or severe injury to the head resulting in long-term and even permanent disability. The recapitulation of this clinical scenario in animal models should allow examination of the pathophysiology of the trauma and its treatment. To date, only a few studies have demonstrated TBI animal models encompassing the three levels of trauma severity. Thus, in the present study we characterized in mice and rats both brain histopathologic and behavioral alterations across a range of injury magnitudes arising from mild, moderate, and severe TBI produced by controlled cortical impact injury technique. Here, we replicated the previously observed TBI severity-dependent brain damage as revealed by 2,3,5-triphenyltetrazolium chloride staining (severe > moderate > mild) in rats, but also extended this pattern of histopathologic changes in mice. Moreover, we showed severity-dependent abnormalities in locomotor and cognitive behaviors in TBI-exposed rats and mice. Taken together, these results support the use of rodent models of TBI as a sensitive platform for investigations of the injury-induced neurostructural and behavioral deficits, which should serve as key outcome parameters for testing experimental therapeutics. (C) 2009 Elsevier B.V. All rights reserved.