Effects of Repeated Lipopolysaccharide Stimulation on the Development of Antigen-presenting Cells and T Cells Pool in Hen Vagina
Effects of Repeated Lipopolysaccharide Stimulation on the Development of Antigen-presenting Cells and T Cells Pool in Hen Vagina
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DOI：
10.2141/jpsa.0120086
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发表时间：
2013-01-01
影响因子：
1.5
通讯作者：
Yoshimura, Yukinori
中科院分区：
文献类型：
--
作者：
Nii, Takahiro;Isobe, Naoki;Yoshimura, Yukinori
本研究的目的是确定T细胞介导的粘膜免疫是如何在阴道内发展的。抗原呈递细胞和T细胞在宿主防御的早期过程中起重要作用。表达CC趋化因子受体（CCR）的成熟T细胞和未成熟树突状细胞相反，未成熟的T细胞和成熟的树突状细胞表达CCR7。研究了反复的抗原刺激是否对阴道内抗原呈递细胞和T细胞池的发育有效。采用生理盐水（对照组）或脂多糖（LPS）在10 d内静脉注射1次（单次注射组）或5次（重复注射组）。最后一次注射后1天收集阴道。冷冻切片对CD4（+）、CD8（+）、TCR γ δ (+) T细胞和MHCⅱ类+细胞进行免疫染色。采用定量RT-PCR分析CCR6、CCR7和MHCⅱ类蛋白的表达。lps重复注射组CD4（+）、CD8（+）、TCR γ δ (+) T细胞频率显著升高，而MHCⅱ+细胞频率无显著变化。lps重复注射组CCR6的表达明显上调，而CCR7和MHCⅱ类的表达不受影响。单次LPS注射和生理盐水注射均不影响各组T细胞亚群和MHCⅱ类+细胞的密度以及趋化因子受体、MHCⅱ类的表达。这些结果表明，反复的抗原刺激可能不会影响抗原提呈细胞的抗原提呈能力，但可能导致T细胞亚群流入阴道。这些积累的T细胞池可能有助于在阴道粘膜中形成宿主免疫的先决条件。
The aim of this study was to determine how the mucosal immunity mediated by T cells is developed in the vagina. Antigen-presenting cells and T cells play important roles in the early process of host defense. Mature T cells and immature dendritic cells expressing CC chemokine receptor (CCR) 6. In contrast, immature T cells and mature dendritic cells express CCR7. It was examined whether repeated antigen stimulation is effective for development of the pool of antigen-presenting cells and T cells in the vagina. White Leghorn hens were intravaginally injected 1 (single injection group) or 5 times (repeated injection group) in 10 days with saline (control) or lipopolysaccharide (LPS). The vagina was collected 1 day after the final injection. Frozen sections of them were immunostained for CD4(+), CD8(+), TCR gamma delta(+) T cells and major histocompatibility complex (MHC) class II+ cells. Expressions of CCR6, CCR7 and MHC class II were analyzed by quantitative RT-PCR. The frequency of CD4(+), CD8(+), TCR gamma delta(+) T cells were significantly increased in LPS-repeated injection group, however that of MHC class II+ cells was not changed significantly. The expression of CCR6 was significantly upregulated in LPS-repeated injection group, however, the expression of CCR7 and MHC class II was not affected. The densities of the each T cell subset and MHC class II+ cells, as well as expressions of chemokine receptor, MHC class II were not affected by single LPS injection or saline injection. These results suggest that the repeated antigen stimulation may not affect antigen-presenting ability by antigen-presenting cells, whereas it may lead influx of T cell subsets in the vagina. These accumulated T cells pool may contribute to form prequiescence of host immunity in the vaginal mucosa.