Lipid analysis of the sex pheromone gland of the moth Heliothis virescens
Lipid analysis of the sex pheromone gland of the moth Heliothis virescens
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DOI：
10.1002/arch.20058
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发表时间：
2005-06-01
影响因子：
2.2
通讯作者：
Foster, SP
中科院分区：
文献类型：
--
作者：
Foster, SP
对烟夜蛾雌蛾性信息素腺体的脂肪酸和脂类含量进行了分析。腺体的基础甲基化分析显示大量的（Z）-11-己癸烯酸甲酯（Z11-16：Acyl），主要信息素组分（Z）-11-己癸烯醛的脂肪酰基类似物，以及少量的（Z）-11-十八烯酸甲酯。还观察到各种常见脂肪酸的甲酯。腺体脂质的HPTLC分析显示大量的三酰基甘油（TGS），以及较少量的1，2-二酰基甘油（1，2-DG）、2-单酰基甘油（2-MG）、磷脂酰乙醇胺和磷脂酰胆碱。这些脂质中Z11-16：酰基的最大量在TG中，在两种磷脂类中的量较少，在其他中性脂质中仅为痕量。提取不同年龄和光周期时间的雌性腺体，通过硅胶SPE分离成中性和极性组分，并测定这些组分中的脂肪酸滴度。对于2日龄雌性，所有脂肪酸（尤其是Z11-16：酰基）在暗期中期均显示出显着的总脂质和中性脂质组分峰;还观察到极性组分中不太明显但显着的Z11-16：酰基峰。然而，在任何时候都只有相对小比例（< 50%）的这种酸从二氧化硅中回收。这种“不可恢复的”Z11-16：酰基在2日龄雌性的暗期中期显示出戏剧性和显著的峰，大致对应于最大信息素滴度。腺体中的所有其他酸均以高比例回收，并且它们各自的“不可回收”滴度在任何分析时间均无差异。基于先前的工作，该不可回收的Z11-16：Acyl可能是CoA酯。因此，看来，信息素腺体的H。绿僵菌保持辅酶A酯和TG形式的Z11-16：酰基库，其可用于信息素的生物合成。当生物合成酶（可能是脂肪酸还原酶）不能足够迅速地利用生物合成的Z11-16：酰基的量时，这些池在最大信息素产生期间最大。(c)2005 Wiley-Liss，Inc.
The sex pheromone gland of female Heliothis virescens was analyzed for fatty acid and lipid content. Base methanalysis of the gland showed a large amount of methyl (Z)-11-hexodecenoate(Z11-16:Acyl), the fatty acyl analog of the major pheromone component, (Z)-11-hexodecenal, as well as a small amount of methyl (Z)-11-octadecenoate. Methyl esters of various common fatty acids were also observed. HPTLC analysis of the glandular lipids revealed large quantities of triacylglycerols (TGS), and lesser amounts of 1,2-diacylglycerols (1,2-DGs), 2-monoacylglycerols (2-MGs), phosphatidyl ethonolamines, and phosphatidyl cholines. The greatest amount of Z11-16:Acyl in these lipids was in the TGs, with lesser amounts in the two phospholipid classes and only trace amounts in the other neutral lipids. The glands of females at various ages and photoperiodic times were extracted, fractionated into neutral and polar fractions by silica SPE, and fatty acid titers in these fractions determined. All fatty acids, but notably Z11-16:Acyl, showed significant total and neutral lipid fraction peaks at mid scotophase for 2-day-old females; a less dramatic, but significant, Z11-16:Acyl peak in the polar fraction was also observed. However, only a relatively small proportion (< 50%) of this acid was recovered from the silica at all times. This "non-recoveroble" Z11-16:Acyl showed a dramatic and significant peak at mid scotophase for 2-day females, corresponding roughly with maximal pheromone titer. All other acids in the gland were recovered in high proportions, and their respective "non-recoverable" titers were not different at any of the times analyzed. Based on previous work, this non-recoverable Z11-16:Acyl is likely the CoA ester. Therefore, it appears that the pheromone gland of H. virescens maintains pools of Z11-16:Acyl in both CoA ester and TG forms, which are available for biosynthesis of pheromone. These pools ore greatest during maximal pheromone production when the biosynthetic enzymes, possibly the fatty acid reductase, are unable to utilize rapidly enough the quantities of Z11-16:Acyl biosynthesized. (c) 2005 Wiley-Liss, Inc.