A highly repeated sequence in the domestic pig: a gender-neutral probe.
A highly repeated sequence in the domestic pig: a gender-neutral probe.
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家猪中高度重复的序列：性别中立探针。
DOI：
10.1093/nar/17.23.10120
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发表时间：
1989
影响因子：
14.9
通讯作者：
R. McGraw
中科院分区：
文献类型：
--
作者：
M. Akamatsu;Z. Y. Chen;P. Dziuk;R. McGraw
我们从家猪(Sus Scrofa)中克隆并测序了一个高度重复的卫星型DNA序列。携带该序列的DNA在基因组DNA的EcoR1酶切中可见一条约1kb的条带。将该条带克隆到pUC9中。现在命名为PM/F-1258的克隆使用M13-双脱氧法进行了测序(图1)。1258bp的序列显示了约44个碱基的不完全内部重复。与克隆片段杂交的序列在公猪和母猪的基因组DNA中都有同样好的表现。我们已经使用克隆的序列作为性别中立的探针来确定胚胎组织样本的性别(图2)。
We have cloned and sequenced a highly repeated satellite-type DNA sequence from the domestic pig (Sus scrofa). DNA bearing this sequence is visible in EcoR1 digests of genomic DNA as a band of approximately 1 kb. This band was isolated and cloned into pUC9. A clone now designated pM/F-1258 was sequenced using M13-dideoxy methods (Figure 1). The 1258 bp sequence shows imperfect intemal repetitions of about 44 bases. Sequences hybridizing to the cloned segment are equally well-represented in the genomic DNA of male and female pigs. We have use the cloned sequence as a gender-neutral probe in determining the sex of embryonic tissue samples (Figure 2).