Quantification of Hepatic Steatosis with 3-T MR Imaging: Validation in ob/ob Mice
Quantification of Hepatic Steatosis with 3-T MR Imaging: Validation in ob/ob Mice
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DOI：
10.1148/radiol.09090131
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发表时间：
2010-01-01
期刊：
影响因子：
19.7
通讯作者：
Reeder, Scott B.
中科院分区：
文献类型：
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作者：
Hines, Catherine D. G.;Yu, Huanzhou;Reeder, Scott B.
目的：为了验证定量成像技术用于检测和测量脂肪变性与磁共振（MR）成像在ob/ob小鼠肝脂肪变性modelsofhepaticsteatosis.Materials和Methods：内部研究动物和资源中心批准了这项研究。28只年龄逐渐增加的雄性ob/ob小鼠接受成像，随后处死。6只ob/+小鼠作为对照动物。脂肪分数成像采用基于化学位移的水脂分离方法。将以下三种常规脂肪定量方法与成像进行比较：脂质提取和定性和定量组织学分析。用脂肪的单峰和多峰光谱模型重建脂肪分数图像，并校正和不校正T2* 效应。结果：脂肪提取、定性和定量组织学分析与脂肪定量成像结果高度相关（r（2）分别为0.92，0.87，0.82）。当图像重建中包括多个脂肪峰和T2* 校正时，成像测量与脂质提取（P= 0.06）或定量组织学（P= 0.07）测量之间无显著差异。重建中，T2* 校正，准确的光谱建模，或两者都被排除产生较低的协议相比，由其他技术产生的结果。成像测量与低脂肪分数小鼠的组织学分级相关性特别好（截距，-1.0% +/-1.2 [标准差]）。结论：MR成像可用于准确定量肝脏脂肪变性动物模型中的体内脂肪，并可作为肝脏脂肪变性的定量生物标志物。(C)RSNA，2010年
Purpose: To validate quantitative imaging techniques used to detect and measure steatosis with magnetic resonance (MR) imaging in an ob/ob mouse model of hepatic steatosis.Materials and Methods: The internal research animal and resource center approved this study. Twenty-eight male ob/ob mice in progressively increasing age groups underwent imaging and were subsequently sacrificed. Six ob/+ mice served as control animals. Fat fraction imaging was performed with a chemical shift-based water-fat separation method. The following three methods of conventional fat quantification were compared with imaging: lipid extraction and qualitative and quantitative histologic analysis. Fat fraction images were reconstructed with single-and multiple-peak spectral models of fat and with and without correction for T2* effects. Fat fraction measurements obtained with the different reconstruction methods were compared with the three methods of fat quantification, and linear regression analysis and two-sided and two-sample t tests were performed.Results: Lipid extraction and qualitative and quantitative histologic analysis were highly correlated with the results of fat fraction imaging (r(2) = 0.92, 0.87, 0.82, respectively). No significant differences were found between imaging measurements and lipid extraction (P=.06) or quantitative histologic (P=.07) measurements when multiple peaks of fat and T2* correction were included in image reconstruction. Reconstructions in which T2* correction, accurate spectral modeling, or both were excluded yielded lower agreement when compared with the results yielded by other techniques. Imaging measurements correlated particularly well with histologic grades in mice with low fat fractions (intercept, -1.0% +/-1.2 [standard deviation]).Conclusion: MR imaging can be used to accurately quantify fat in vivo in an animal model of hepatic steatosis and may serve as a quantitative biomarker of hepatic steatosis. (C) RSNA, 2010