Lactobacillus plantarum ZS2058 produces CLA to ameliorate DSS-induced acute colitis in mice
Lactobacillus plantarum ZS2058 produces CLA to ameliorate DSS-induced acute colitis in mice
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植物乳杆菌 ZS2058 产生 CLA 以改善 DSS 诱导的小鼠急性结肠炎
DOI：
10.1039/c5ra24491a
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发表时间：
2016-01-01
期刊：
影响因子：
3.9
通讯作者：
Chen, Yong Q.
中科院分区：
文献类型：
--
作者：
Wang, Juntong;Chen, Haiqin;Chen, Yong Q.
植物乳杆菌ZS2058是体外共轭亚油酸（CLA）的高效生产者。为了研究L. plantarum ZS2058是否在体内产生CLA并通过CLA发挥有益作用，采用葡聚糖硫酸钠（DSS）诱导C57BL6/J小鼠急性结肠炎模型。造模前7 d分别给予植物乳杆菌ZS2058、植物乳杆菌ST-III、CLA或代药，造模结束7 d。与植物L. plantarum ST-III相比，植物L. plantarum ZS2058显著抑制了结肠炎小鼠疾病活性指数（DAI）、结肠缩短和髓过氧化物酶活性的升高。植物L. plantarum ZS2058处理改善了组织损伤，保护了结肠粘膜层的完整性，显著降低了促炎细胞因子（tnf - α、IL-1 β、IL-6）的表达，上调了结肠抗炎细胞因子IL-10和核受体PPAR γ的表达。此外，L. plantarum ZS2058处理后，结肠CLA浓度显著升高，这表明L. plantarum ZS2058通过局部产生CLA来预防结肠炎。
Lactobacillus plantarum ZS2058 is an efficient producer of conjugated linoleic acid (CLA) in vitro. To investigate whether L. plantarum ZS2058 produces CLA in vivo and exerts beneficial effects through CLA, an acute colitis model was induced with dextran sodium sulfate (DSS) in C57BL6/J mice. The mice were treated with L. plantarum ZS2058, L. plantarum ST-III, CLA, or vehicle 7 days before modeling until the end of modeling which lasts for seven days. Compared to L. plantarum ST-III, L. plantarum ZS2058 significantly inhibited the increase of the disease activity index (DAI), colon shortening and myeloperoxidase activity in colitic mice. L. plantarum ZS2058 treatment improved the histological damage, protected the colonic mucous layer integrity and significantly attenuated the expression of pro-inflammatory cytokines (TNF-alpha, IL-1 beta, IL-6), while up-regulated the expression of colonic antiinflammatory cytokine IL-10 and nuclear receptor PPAR gamma. Furthermore, colonic CLA concentrations were significantly increased in response to L. plantarum ZS2058 treatment, which demonstrates that L. plantarum ZS2058 prevents colitis via producing CLA locally.