Renin distribution in the rabbit renal microvasculature.
Renin distribution in the rabbit renal microvasculature.
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肾素在兔肾微血管中的分布。
DOI：
10.1161/01.hyp.19.2_suppl.ii36
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Carretero,OA
中科院分区：
文献类型：
--
作者：
Juncos,LA;Ito,S;Nobiling,R;Carretero,OA
免疫细胞化学研究表明，通常位于肾小球传入小动脉的肾素，在慢性刺激肾素-血管紧张素系统期间，也可在小叶间动脉上游发现。我们评估了肾素分布沿着肾脏微血管使用定量分析和免疫细胞化学在兔子，接受了正常钠饮食（0.48%氯化钠），低钠饮食（0.04%氯化钠），或依那普利（1毫克/公斤/天）4周。我们从外皮质显微解剖了1）从小叶间动脉延伸至距肾小球50微米点的传入小动脉（p-AF）的近端部分，2）包括其完整末端（d-AF）在内的远端50微米部分，以及3）不含血管极（GL）的肾小球，并测量了它们的肾素含量。在对照组中，p-AF、d-AF和GL中的肾素分别为0.3 +/- 0.2、27.0 +/- 5.2和2.8 +/- 0.5 ng血管紧张素I/hr/小动脉（或GL）。低钠饮食和依那普利增加了d-AF中的肾素（分别为53.1 +/- 6.9和68.4 +/- 8.1），但没有增加GL中的肾素（3.3 +/- 1.0和3.6 +/- 0.7）。在p-AF中，两者均引起小幅增加（delta = 1.5）;然而，与d-AF的大幅增加（delta = 41）相比，这种增加微不足道。（250字处删节）
Immunocytochemical studies have shown that renin, which is normally located in the juxtaglomerular afferent arteriole, may also be found farther upstream toward the interlobular artery during chronic stimulation of the renin-angiotensin system. We assessed the renin distribution along the renal microvasculature using both quantitative analysis and immunocytochemistry in rabbits that received a normal sodium diet (0.48% NaCl), a low sodium diet (0.04% NaCl), or enalapril (1 mg/kg/day) for 4 weeks. From the outer cortex we microdissected 1) the proximal portion of the afferent arteriole (p-AF) extending from the interlobular artery to a point 50 microns from the glomerulus, 2) the distal 50 microns including its intact terminus (d-AF), and 3) the glomerulus without the vascular pole (GL) and measured their renin content. In controls, renin was 0.3 +/- 0.2, 27.0 +/- 5.2, and 2.8 +/- 0.5 ng angiotensin I/hr/arteriole (or GL) in the p-AF, d-AF, and GL, respectively. The low sodium diet and enalapril increased renin in the d-AF (53.1 +/- 6.9 and 68.4 +/- 8.1, respectively) but not in the GL (3.3 +/- 1.0 and 3.6 +/- 0.7). In the p-AF, both caused a small increase (delta = 1.5); however, this increase was minuscule compared with the large increase in the d-AF (delta = 41).(ABSTRACT TRUNCATED AT 250 WORDS)