Pulmonary surfactant proteins A and D enhance neutrophil uptake of bacteria
Pulmonary surfactant proteins A and D enhance neutrophil uptake of bacteria
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DOI：
10.1152/ajplung.1998.274.6.l958
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发表时间：
1998-06-01
影响因子：
4.9
通讯作者：
Sastry, KN
中科院分区：
文献类型：
--
作者：
Hartshorn, KL;Crouch, E;Sastry, KN
胶原凝集素是存在于血清和肺分泌物中的一类胶原凝集素蛋白[肺表面活性蛋白（SP）A和SP-D]，据信其参与对各种病原体的先天免疫应答。使用流式细胞术和荧光显微镜检测，SP-A和SP-D显示增加大肠杆菌，肺炎链球菌和金黄色葡萄球菌的钙依赖性中性粒细胞摄取。有证据表明，胶原凝集素通过涉及细菌聚集和对中性粒细胞的直接作用的机制增强细菌摄取。SP-D制剂的多聚化程度是增强细菌摄取的聚集活性和效力的关键决定因素。SP-D和SP-A的调理活性的机制在重要方面不同于调理抗体的那些。这些结果提供了第一个证据表明，表面活性剂凝集素可以促进嗜酸性粒细胞介导的清除细菌在肺中独立的调理抗体。
The collectins are a class of collagenous lectin proteins present in serum and pulmonary secretions [pulmonary surfactant protein (SP) A and SP-D] that are believed to participate in innate immune responses to various pathogens. With the use of flow cytometric and fluorescent-microscopic assays, SP-A and SP-D were shown to increase calcium-dependent neutrophil uptake of Escherichia coli, Streptococcus pneumoniae, and Staphylococcus aureus. Evidence is provided that the collectins enhanced bacterial uptake through a mechanism that involved both bacterial aggregation and direct actions on neutrophils. The degree of multimerization of SP-D preparations was a critical determinant of both aggregating activity and potency in enhancing bacterial uptake. The mechanisms of opsonizing activity of SP-D and SP-A differed in important respects from those of opsonizing antibodies. These results provide the first evidence that surfactant collectins may promote neutrophil-mediated clearance of bacteria in the lung independently of opsonizing antibody.