Carbon nanotube-based DNA biosensor for monitoring phenolic pollutants
Carbon nanotube-based DNA biosensor for monitoring phenolic pollutants
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DOI：
10.1007/s00604-009-0154-2
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发表时间：
2009-04
影响因子：
5.7
通讯作者：
Yanqiong Zheng;Changzhu Yang;Wen-hong Pu;Jingdong Zhang
中科院分区：
文献类型：
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作者：
Yanqiong Zheng;Changzhu Yang;Wen-hong Pu;Jingdong Zhang
将DNA固定在分散在Nafion中的多壁碳纳米管（MWNTs）修饰的玻璃碳（GC）电极上（DNA/MWNTs/GCE），构建了DNA生物传感器。DNA修饰电极表现出两个明确的氧化峰，分别对应于DNA的鸟嘌呤和腺嘌呤残基。研究了用于DNA固定的MWNTs的吸附电位、DNA浓度和数量，以及缓冲液、pH和扫描速率对DNA伏安行为的影响。苯酚、间甲酚和儿茶酚由于与DNA的相互作用，对电极的反应表现出明显的抑制作用。这些发现被用于设计对这些酚类污染物具有线性响应的生物传感器。
A DNA biosensor was constructed by immobilizing DNA on a glassy carbon (GC) electrode modified with multiwall carbon nanotubes (MWNTs) dispersed in Nafion (DNA/MWNTs/GCE). The DNA-modified electrode exhibited two well-defined oxidation peaks corresponding to the guanine and adenine residues of DNA, respectively. The effects of the adsorption potential, DNA concentration and quantity of MWNTs used for DNA immobilization were investigated, as were the effects of buffer, pH and scan rate on the voltammetric behavior of DNA. Phenol,m-cresol and catechol showed noticeable inhibition towards the response of the electrode due to their interactions with DNA. These findings were used to design biosensors with linear response to these phenolic pollutants.