CLONAL RELATEDNESS OF SHIGA-LIKE TOXIN-PRODUCING ESCHERICHIA-COLI O101 STRAINS OF HUMAN AND PORCINE ORIGIN
CLONAL RELATEDNESS OF SHIGA-LIKE TOXIN-PRODUCING ESCHERICHIA-COLI O101 STRAINS OF HUMAN AND PORCINE ORIGIN
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DOI：
10.1128/jcm.33.12.3174-3178.1995
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发表时间：
1995-12-01
影响因子：
9.4
通讯作者：
KARCH, H
中科院分区：
文献类型：
--
作者：
FRANKE, S;HARMSEN, D;KARCH, H
近年来，研究发现产志贺样毒素大肠杆菌（SLTEC）O 101与人类出血性结肠炎和溶血性尿毒症综合征有关。在这项研究中，SLTEC O 101菌株从人类和猪的特点是克隆相关性的核苷酸序列分析，其slt基因，DNA指纹图谱的基因组DNA，并确定毒力因子。5株E. coliO 101菌株的克隆和序列测定。对于所有菌株，推导的B亚基的氨基酸序列与引起猪水肿病的经典SLTEC O 139菌株中存在的SLT-Ile的氨基酸序列相同。A亚基与SLT-Ile的同源性超过99%。DNA指纹图谱分析显示人与猪O 101分离株之间存在高度的遗传相关性。大肠杆菌溶血素）。不存在典型的产生SLT-Ⅰ和SLT-Ⅱ的E.大肠杆菌中发现的毒素SLT-Ile，以前只在猪大肠杆菌中发现。coli，提示了大肠杆菌的一种新的致病机制。大肠杆菌O 101感染人类。出于诊断目的，我们建议使用与slt-Ile互补的PCR引物和DNA探针来正确识别此类菌株并进一步评估其在人类疾病中的作用。
Shiga-like toxin (SLT)-producing Escherichia coli (SLTEC) O101 has recently been associated with hemorrhagic colitis and hemolytic-uremic syndrome in humans. In this study, SLTEC O101 strains from humans and pigs were characterized for clonal relatedness by nucleotide sequence analysis of their slt genes, DNA fingerprinting of genomic DNA, and determination of virulence factors. The slt genes of five E. coli O101 strains were cloned and sequenced. For all strains, the deduced amino acid sequences of the B subunits were identical to those of the SLT-Ile present in the classical SLTEC O139 strains that cause edema disease in pigs. The A subunit revealed more than 99% homology to that of SLT-Ile. DNA fingerprinting revealed a high degree of genetic relatedness between the human and porcine O101 isolates, None of the O101 strains investigated had virulence factors frequently found in porcine (F107 fimbriae or heat-stable or heat-labile enterotoxins) or human SLTEC strains (eaeA or enterohemorrhagic E. coli hemolysin). The absence of virulence factors typical of SLT-I- and SLT-II-producing E. coli together with the presence of SLT-Ile, a toxin previously seen only in porcine E. coli, suggests a new pathogenic mechanism for E. coli O101 infection of humans. For diagnostic purposes, we recommend the use of PCR primers and DNA probes complementary to slt-Ile to correctly identify such strains and to further evaluate their role in human diseases.