A transmembrane glycoprotein, gp38, is a novel marker for immature hepatic progenitor cells in fetal mouse livers.
A transmembrane glycoprotein, gp38, is a novel marker for immature hepatic progenitor cells in fetal mouse livers.
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DOI：
10.1007/s11626-010-9354-7
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发表时间：
2011-01
影响因子：
2.1
通讯作者：
Uemoto, Shinji
中科院分区：
文献类型：
--
作者：
Konishi, Sayuri;Yasuchika, Kentaro;Ishii, Takamichi;Fukumitsu, Ken;Kamo, Naoko;Fujita, Naoya;Ikai, Iwao;Uemoto, Shinji
以前，我们阐明了胎肝组织中肝祖细胞（HPC）的表面抗原谱，即CD 49 f + CD 45 − Thy 1 −细胞部分。然而，这些细胞是含有不同分化阶段的异质细胞群。本研究旨在使用一种新的表面抗原gp 38检测更多的未成熟HPC。从E13.5至E18.5胎鼠收获胎肝，胶原酶消化后，获得HPC，并使用流式细胞术将其分为两个亚群：gp 38阳性HPC和gp 38阴性HPC。用免疫细胞化学和RT-PCR对两种类型的HPC进行鉴定。用BrdU掺入法和MTS法检测细胞增殖活性。并利用基因芯片技术对基因表达进行了全面的研究。两种类型的HPC表达甲胎蛋白。然而，来自E13.5胎肝的gp 38阳性HPC不表达白蛋白或细胞角蛋白19，而gp 38阴性HPC则表达。基因芯片检测结果显示，在gp 38阳性的HPCs中，Wnt信号通路相关基因表达上调。此外，Wnt 3a对gp 38阳性HPC具有增殖作用。总之，gp 38-阳性HPC来自胎肝组织，直到E13.5，因此可以在胎肝干细胞的候选人。
Previously, we clarified the surface antigen profiles of hepatic progenitor cells (HPCs) in fetal liver tissue as the CD49f+CD45−Thy1− cell fraction. However, these cells were a heterogeneous cell population containing various stages of differentiation. This study aimed to detect more immature HPCs, using a novel surface antigen, gp38. After the collagenase digestion of fetal livers harvested from E13.5 to E18.5 fetal mice, HPCs were obtained and divided into two subpopulations using flow cytometry: gp38-positive HPCs, and gp38-negative HPCs. Both types of HPCs were characterized by immunocytochemistry and RT-PCR. The proliferative activity was compared by BrdU incorporation and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTS) assay. Furthermore, the comprehensive gene expression was investigated by DNA microarray. Both types of HPCs expressed alpha-fetoprotein. However, the gp38-positive HPCs derived from E13.5 fetal livers did not express albumin or cytokeratin 19, while the gp38-negative HPCs did. DNA microarray revealed that some genes related to the Wnt signal pathway were up-regulated in the gp38-positive HPCs. Furthermore, Wnt3a had a proliferative effect on the gp38-positive HPCs. In conclusion, the gp38-positive HPCs derived from fetal liver tissue until E13.5 could therefore be candidates for hepatic stem cells in the fetal liver.
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DOI：
10.1083/jcb.200108066
发表时间：
2002-01-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Suzuki A;Zheng YW;Kaneko S;Onodera M;Fukao K;Nakauchi H;Taniguchi H
通讯作者：
Taniguchi H
影响因子：
8.8
作者：
通讯作者：
--
影响因子：
29.4
作者：
Crosby, HA;Kelly, DA;Strain, AJ
通讯作者：
Strain, AJ
影响因子：
13.5
作者：
Oertel, M;Rosencrantz, R;Shafritz, DA
通讯作者：
Shafritz, DA
影响因子：
3.7
作者：
Ishii, T;Yasuchika, K;Ikai, I
通讯作者：
Ikai, I