Quantitative immunocytochemical analysis of Mn SOD in alveolar type II cells of the hyperoxic rat.
Quantitative immunocytochemical analysis of Mn SOD in alveolar type II cells of the hyperoxic rat.
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高氧大鼠肺泡 II 型细胞中 Mn SOD 的定量免疫细胞化学分析。
DOI：
10.1152/ajplung.1994.267.4.l475
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Crapo,JD
中科院分区：
文献类型：
--
作者：
Vincent,R;Chang,LY;Slot,JW;Crapo,JD
我们分析了锰超氧化物歧化酶（Mn SOD）蛋白表达的幅度和均匀性在肺泡II型细胞暴露于高氧使用定量胶体金免疫细胞化学和形态计量技术。将Sprague-Dawley大鼠连续暴露于正常空气或85%氧气中7天和14天。通过气管内滴注多聚甲醛-戊二醛固定剂固定肺。将肺样品在乙醇中脱水并包埋在LR-White中。电镜切片用抗大鼠Mn SOD兔抗血清标记，然后用蛋白A金标记。测定亚细胞区室的标记密度（金颗粒/微米2），并使用随机点覆盖测量相对细胞器体积。结果证实，肺泡II型细胞是高氧大鼠肺中Mn SOD反应的一个位点，并通过以下变化的组合促进这种反应：线粒体基质中Mn SOD浓度的增加，每个细胞的线粒体体积的增加，和II型细胞增生。
We have analyzed the magnitude and uniformity of the expression of manganese superoxide dismutase (Mn SOD) protein in alveolar type II cells of rats exposed to hyperoxia using quantitative colloidal gold immunocytochemistry and morphometric techniques. Sprague-Dawley rats were exposed continuously to normal air or to 85% oxygen for 7 and 14 days. The lungs were fixed by intratracheal instillation of a paraformaldehyde-glutaraldehyde fixative. Lung samples were dehydrated in ethanol and embedded in LR-White. Thin sections for electron microscopy were labeled with anti-rat Mn SOD rabbit antiserum followed by protein-A gold. The labeling density (gold particles/micron 2) over subcellular compartments was determined, and relative organelle volumes were measured using a random point overlay. The results confirm that alveolar type II cells are a locus of Mn SOD response in the lungs of hyperoxic rats and contribute to this response through a combination of changes: an increase of Mn SOD concentration in the mitochondrial matrix, an increase of mitochondrial volume per cell, and type II cell hyperplasia.